A retrospective study on qualitative assessment of copper content in oral leukoplakia, submucous fibrosis, and squamous cell carcinoma with rhodamine stain
Journal Title: Journal of Interdisciplinary Histopathology - Year 2015, Vol 3, Issue 3
Abstract
Background: Trace elements are required in small concentrations as essential active components of biological enzyme systems. They are in the form of metalloenzymes and copper is one such component. Copper metabolism is profoundly altered in neoplastic disease. Copper plays an important role in tumor angiogenesis especially in early stages and is also necessary for endothelial cell activation. Copper content in tissues of oral leukoplakia (OL), oral submucous fibrosis (OSMF), and oral squamous cell carcinoma (OSCC) has not been studied extensively. Hence, we attempted this retrospective study to analyze copper content in the tissues by using rhodamine stain for better understanding of pathogenesis. Aim and Objectives: To evaluate and compare the copper content and its depth of penetration in tissue sections of diagnosed cases of oral precancer and cancer by using rhodamine staining method. Materials and Methods: This retrospective analytical study was conducted on histologically diagnosed 10 cases each of OL, OSMF, and OSCC. Ten cases of normal mucosa served as control. The selected cases were subjected to staining with rhodamine for qualitative analysis of copper content. Results: Paired t-test showed a significant increase in copper content in the study group (P = 0.516) as compared to control group. Krushall–Wallis non-parametric test showed higher mean value of copper content in cases of OSMF (2.00) than the cases of OL (1.20) and OSCC (1.70). Conclusion: Copper content by rhodamine staining technique was found to be higher in OSMF than OL and OSCC this staining technique can be used as a prognostic indicator for assessment of disease progression.
Authors and Affiliations
Priya Joshi, Bhagyalaxmi Hongal, Neha Agnihotri, Rutuja Kempwade
Keywords
Related Articles
- EP ID EP111750
- DOI 10.5455/jihp.20150717124034
- Views 87
- Downloads 0
Giant cystic retroperitoneal lymphangioma in an adult
Lymphangioma is an uncommon congenital malformation of the lymphatic system affecting usually the younger age group. These tumours frequently affect the neck and axillary region. Retroperitoneal lymphangiomas are rare an...
Xanthogranulomatous Cholecystitis Masquerading As Gallbladder Cancer: A Diagnostic Dilemma
Xanthogranulomatous cholecystitis is an uncommon variant of chronic cholecystitis, characterized by marked thickening of the gallbladder wall and dense local adhesions that may be confused with a malignant process. In th...
Cadmium-Induced Testicular Toxicity, Oxidative Stress and Histopathology in Wistar Rats: Sustained Effects of Polyphenol-Rich Extract of Vernonia Amygdalina (Del.) Leaf
Background: Cadmium (Cd) is a toxic heavy metal of both environmental and occupational concerns. The health impact of ethno-botanical approaches in attempts to ameliorate its deleterious effects in biological systems sho...
Blastic Plasmacytoid Dendritic Cell Neoplasm (BPDCN): A Case of Multiple, Painless, Erythematous Nodule on the Back
Blastic plasmacytoid dendritic cell neoplasm is a rare, highly aggressive hematopoietic malignancy that is characterized by the clonal proliferation of immature or precursors of plasmacytoid dendritic cells. We report...
Primary Well Differentiated Squamous Cell Carcinoma of Kidney: A Rare Entity
Cancers of the kidney and renal pelvis are the ninth most common malignant cancer and form the 12th most common cause of all cancer-related deaths. Among malignant renal tumors, squamous cell carcinoma is rare neoplasm a...