A REVIEW ON CURRENT TRENDS AND ADVANCEMENTS IN BIOANALYTICAL METHODS
Journal Title: Int J of Pharm Res & Analy - Year 2014, Vol 4, Issue 6
Abstract
This paper reviews the recent developments in bio-analysis sample preparation techniques and chromatographic techniques give an update on basic principles and a comparative discussion on the benefits and limitation of every technique. Any bio-analytical technique includes several steps, all of them being vital in order to attain reliable results. The first step is taking aliquots of samples for the analysis, followed by the extraction procedure and sample clean-up, chromatographic analysis and detection. Conventional sample preparation techniques including solid phase extraction (SPE), liquid-liquid extraction (LLE), protein precipitation (PP) and many modern approaches including molecularly imprinted polymers (MIP), solid phase micro extraction (SPME), liquid-liquid micro extraction (LLME), micro extraction by packed sorbent (MEPS) and plenty of others have additionally been featured as fundamental and significant step of bio-analytical methods. Current trends in fast liquid chromatographic separations involve monolith technology, high temperature liquid chromatography (HTLC) and ultra-high performance liquid chromatography (UHPLC). UHPLC has recently become a wide-spread analytical technique in many laboratories which focus on fast and sensitive bio-analytical assays. The key advantages of UHPLC are the increased speed of analysis, higher separation efficiency and resolution, higher sensitivity and much lower solvent consumption as compared to other analytical approaches. This is all enabled by specially designed instruments and sub-2-microne particle packed analytical columns
Authors and Affiliations
Vidya K*, , Uma Maheshwara Rao V , Ajitha A
Keywords
Related Articles
- EP ID EP106589
- DOI -
- Views 123
- Downloads 0
UTILITY OF ORAL AND VAGINAL MISOPROSTOL IN THE INDUCTION OF LABOUR: A COMPARATIVE STUDY
This study was conducted to compare the efficiency and safety of oral (50 μg) and vaginal (25 μg) misoprostol for labour induction. In this study one Hundred patients with indications for labour induction randomly...
EFFECT OF USING BLACK PEPPER AS NATURAL FEED ADDITIVE ON PERFORMANCE AND CARCASS QUALITY OF BROILER CHICKS
Flowcytometry is a powerful technology mainly used for investigations in cell biology and isolation of individual cells.It is a laser based biophysical analytical technique used mainly for cell sorting, cell counti...
ANALYTICAL STUDY OF PROCESSING UTILITY AND SOME PHYSICO-CHEMCIAL PARAMETERS OF RAW WATER, SOFT WATER AND DEMINERALIZED WATER IN KORBA AREA
World Water & Environmental Engineering is an international water quality magazine that reaches over 10,000 water quality professionals in Central India and South India. Helmed by London-based publisher Nic Chr...
RECENT APPROCHES FOR IMPURITY PROFILING IN PHARMACEUTICAL FORMULATIONS
In the pharmaceutical world, an impurity is considered as any other organic material, besides the drug substance, or ingredients, arise out of synthesis or unwanted chemicals that remains with API’s. The impurity m...
DEVELOPMENT AND VALIDATION OF NEW SPECTROPHOTOMETRIC METHODS FOR THE QUANTITATIVE ESTIMATION OF SILODOSIN IN BULK DRUG AND PHARMACEUTICAL FORMULATIONS
Three simple, sensitive, accurate and rapid spectrophotometric methods A, B and C have been developed for the quantitative estimation of Silodosin in bulk drug and also pharmaceutical formulations. Method A is a UV...