A Screen House Experiment to Evaluate the Biostimulation Potentials of Cow Dung on a Crude Oil Polluted Soil
Journal Title: International Journal of Agriculture Innovations and Research - Year 2018, Vol 6, Issue 5
Abstract
This screen house study was conducted to investigate the effect of the application of cow dung to crude oil polluted soils. Four rates of cow dung (0, 1, 2 and 3g) and four rates (0, 5, 10 and 15ml) of crude oil per 10kg of soil were used, a total of sixteen (16) treatment combinations were applied. Each treatment was replicated three times, for a total of forty eight (48) pots. The rate used is equivalent to 0, 200, 400 and 600kg/ha and 0, 1000, 2000 and 3000 litres/ha of cow dung and crude oil respectively. The experiment was laid out in a completely randomized designed. Bacteria, fungi and Physico-chemical properties of the soils were determined before pollution, two weeks after pollution and at the end of the experiment. The results for the physico-chemical properties of soil indicate an increased in pH, carbon to nitrogen ratio (C:N), total organic carbon (TOC) and nitrogen (N) while Phosphorus (P) decreased as the level of crude oil increased despite remediation with cow dung. There was an increase in bacterial count for both the control and the treatment groups. The result range from 1.3 X104 to 28.6 X104. The genus of bacteria identified were Pseudomonas, Bacillus, Micrococcus, Proteus, Clostridium and Nocardia species. Four isolates were gram positive while 2 were gram negative. Five were rod-shaped while one was in coccus form, while the fungi isolates are Cladosporium, Pichia, Aspergillus, Fusarium species. Generally remediation for the oil contaminated soil at the end of tenth week revealed a positive correlation coefficient in the degree of remediation during the trial periods
Authors and Affiliations
Oriakpono Obemeata, et al.
Keywords
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