A simple and reliable method for obtaining monoconidial culture and storage of Magnaporthe oryzae
Journal Title: International Journal of Life Science - Year 2018, Vol 6, Issue 2
Abstract
Monoconidial isolates of Magnaporthe oryzae were successfully cultured and maintained using a simple, reliable and inexpensive isolation procedure developed by us. It can also be performed with minimum equipment. This procedure will be helpful for researchers to save their time in standardizing the isolation and storage. We have isolated 72 M. oryzae monoconidial isolates from different agro climatic regions of Karnataka, India and pure cultures were stored in filter papers, in our investigation. The current procedure is useful in carrying out research investigation requiring genetically uniform cultures.
Authors and Affiliations
Jagadeesh D, Prasanna Kumar MK, Devaki NS
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