A Simple Method to Purify Growth and Milk Enhancing Hormones (Growth Hormone and Prolactin) In High Yield From the Pituitary Glands of Indian Domestic Goats (Capra hircus)
Journal Title: IOSR Journal of Pharmacy (IOSRPHR) - Year 2018, Vol 8, Issue 4
Abstract
: Pituitary hormones GH and PRL have been used as external stimulating agents in farm animals to increase the yield of milk and meat. Here, a simple, protocol for purification of Growth Hormone (GH) and Prolactin (PRL) from the pituitary glands of Indian domestic goats (Capra hircus) has been described. Extraction of pituitaries with ammonium sulphate coupled with QAE-Sephadex anion exchange chromatography provided purified GH. The GH preparation was found to be pure in SDS-PAGE analysis and immuno-reactive to anti-buffalo GH antibody in ELISA. QAE-Sephadex was also useful in fractionating goat GH into different charge isoforms. PRL was purified from an alcoholic extract by gel-filtration chromatography on S-200.The PRL preparation was found to be pure in SDS-PAGE analysis. The respective yields of pure GH and PRL were 4.9 and 2.9 grams per kilogram of pituitaries. Molecular weights of major form of GH and PRL as determined SDS-PAGE was found to be 20 and 23 kDa respectively. Physico-chemical properties of goat hormones were found to be similar to the buffalo hormones. Domestic goat is an important source of farm products (milk and meat) in many developing countries. The yield of products can be improved on per animal basis by administering purified homologous GH and PRL. For this purpose, a simple yet efficient method to get pure preparations of GH and PRL, as described in the present communication may be of great importance. Also, the purified material obtained can be used as standard for various immunoassay and bioassay experiments.
Authors and Affiliations
Rajesh Chaudharyab, Manoj Panchala, K. Muralidhara
Keywords
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