A STIMULATORY APPROACH FOR IN VITRO ACTIVATION OF HUMAN SPERM MOTILITY BY USING THEOPHYLLINE.
Journal Title: International Journal of Advanced Research (IJAR) - Year 2018, Vol 6, Issue 4
Abstract
Background: In developed countries, about (2?4%) of births involve the using of assisted reproductive technologies (ART\'s). In these technologies before using semen samples for insemination firstly it must be processed and specifically sperm preparation methods and materials used to improve sperm functions especially motility in order to ensure increasing in the successful of pregnancy rates next ART\'s. Theophylline pharmacological agents recently commonly used in stimulating motility in human sperm thus by increasing intracellular levels of cyclic adenosine 3?, 5?-monophosphate (cAMP), a molecule involved in formation of sperm energy, which is result of inhibitory properties on phosphdiesterase (PDE) function. Objective: To evaluate whether in vitro sperm activation by Theophylline improves sperm functional parameters as compared with non-activated semen samples. Subjects, Materials and Methods: Sixty male were participated in this study, twenty Normozoospermic subjects and forty Asthenozoospermic patients. Semen samples were collected and seminal fluid analysis was done according to (WHO 1999,2010) guidelines. Each sample was divided equally into three portions, centrifugation was done to remove seminal plasma from all, and then Swim-up technique was dependent for in vitro sperm activation by using FertiCult Flushing medium, Theophylline solution and SpermMobil dilution respectively. Sperm parameters were measured in each activated portion according to (WHO 1999) guidelines. Results: Activation of semen sample groups by using of FertiCult Flushing medium, Theophylline solution and SpermMobil dilution in this study resulted in significant reduction in sperm concentration and highly significant improvement (P<0.001) in the percentage of sperm parameters in comparison with non-activated ones. However, SpermMobil dilution resulted in least reduction in sperm concentration and best result of sperm motility and morphologically normal sperm (MNS). Conclusion: From all comparisons between activation media which have been used in this study with non-activated semen, whatever semen is activated, concentration of sperm will be decreased. Sperm motility and MNS will be improved after activation by FertiCult medium, Theophylline solution and SpermMobil dilution, best results were in increases of sperm motility and MNS which was attributed to SpermMobil dilution.
Authors and Affiliations
Huda S. Abbas, Hayder A. L. Mossa, Lubna A. Al-Anbari, Balsam A. Al-Zahawi.
Keywords
Related Articles
- EP ID EP290529
- DOI 10.21474/IJAR01/6961
- Views 49
- Downloads 0
PROMOTING TOURISM IN NIGERIA THROUGH AN ENHANCED TOURISM MANAGEMENT SYSTEM - A PRELIMINARY STUDY.
In an era where information plays a vital role in our society because its use is unavoidable inour day-to-day activities; tourism has become popular as a leisure way of travel and it relies on informationtechnologyinorde...
A CASE OF HEMOPTYSIS DUE TO SILICOTUBERCULOSIS: CASE REPORT.
A 60year old gentleman, mason by occupation, presented with shortness of breath since 9months, cough since 5months, streaky hemoptysis since 5days. He is a smoker with 20 pack-years. On examination patient is tachypneic...
COMPARATIVE EVALUATION OF THE AXIS MODIFICATION, CENTERING ABILITY AND APICAL DEBRIS EXTRUSION USING SINGLE-FILE RECIPROCATING AND CONTINUOUS ROTARY INSTRUMENTATION SYSTEMS IN CURVED ROOT CANALS IN VITRO STUDY.
Aim- The purpose of this study was to study the amount of apically extruded debris and axis modification and centering ability using CBCT in relatively curved root canals with Wave One and One Shape single file systems....
ASSESSMENT OF LANGUAGE DELAY AMONG 0-3 YEAR CHILDREN ATTENDING THE WELL BABY CLINIC IN A TERTIARY CARE CENTRE USING LEST (0-3 YEARS).
The study is a cross sectional study of 200 children conducted using Language Evaluation Scale Trivandrum (LEST 0-3) in the age group birth to 3 years to assess the prevalence of language delay in children attending Well...
LOCAL CONTENT INITIATIVES AND LOCAL DEVELOPMENT: THE TRENDS AND CONSTRAINTS OF THE MINING SECTOR IN CAMEROON.
Unequivocally, the paper seeks to provide a periscopic view of the designs and interventions of local content Initiatives (LCIs) in the mining sector. Indeed, from the review and analysis, the research finds that resource...