A study of Bcl-2 and p-53 immunostaining expressions in colonic carcinomas

Journal Title: MRIMS Journal of Health Sciences - Year 2016, Vol 4, Issue 1

Abstract

Background: Colorectal cancer burden has been steadily rising in women. It was the fourth most common cancer in 1975 and has reached the second position by 1990, with about 49% increase in the number of cases globally over a period of 15 yrs. From 1999–2008, the rate of people dying from colorectal cancer has varied, depending on their race and ethnicity. Objective: To study Bcl-2 and p-53 immunostaining expressions in colonic carcinomas. Methods: The present study is both a retrospective and a prospective study. The study is based on histomorphological evaluation of 30 resected specimen of Colorectal Carcinomas received at the department of Pathology, Osmania General Hospital in a 2 year period from June 2009 to May 2011. The most representative block for all 30 cases was then selected for immune histochemical analysis of p-53 protein and Bcl-2 Oncoprotein for p-53 immunostaining, we used the DO-7 monoclonal antibody (dilution 1:30; DAKO), and, for Bcl-2, the monoclonal antibody 124 was used (dilution 1:80; DAKO). Results: Positive staining for p-53 protein was detected in 56.67% ( 17/30 ) of colorectal adenocarcinomas. There is statistically no significant association observed between p53 expression and Dukes’ stage, TNM stage or Histological grade (p value > 0.05). Similarly no association was found between expression of p-53 and other patient variables, such as age, sex, location of tumor, & tumor differentiation. In the present study statistically there is no significant association between Bcl-2 & p-53 either individually or combined together with any of the parameters (like age, sex, tumor location, Dukes’ stage, Histological grade & TNM stage). Conclusion: Though not statistically significant, to comment as the optimism expressed by certain investigators due to the low number of cases taken up for the present study, a large study group is needed to comment on the prognostic significance of the Bcl-2 & p-53 immuno profile individually and in a combined variation to predict the clinical outcome.

Authors and Affiliations

Shashi Kiran K, Hari Shanker V, Triveni Bhopal, Kandukuri Mahesh Kumar

Keywords

Related Articles

Radiological profile of patients with Cervical Spondylosis

Background: Cervical spondylosis is a common disease of advancing age arising form the effects of a progressive degenerative changes affecting single or multiple inter vertebral disco and consequent hypertrophy, spurring...

A Case Report of the Dual Infection with HAV and HEV in A Pediatric Patient at Lotus Hospital, Hyderabad

Acute viral hepatitis is a major public health problem and is important cause of morbidity and mortality.HAV and HEV are the enterically transmitted viruses, resulting in acute viral hepatitis in the developing countries...

An Analysis of Post Caesarean Section Surgical Site Infections in a Tertiary Care Women’s and Neonate’s Hospital

Background: Surgical Site Infections [SSI] are infections that develop within thirty days of surgery. They are an important cause of Hospital Acquired Infections [HAI]. The Hospital Infection Control Committee [HICC] of...

Cytodiagnosis of medullary carcinoma thyroid- role of FNAC and serum calcitonin- A case report

Abstract: Medullary carcinoma of thyroid (MCT) is a rare neuro-endocrine thyroid malignancy, which is associated with specific supportive diagnostic markers. Despite this, its cytological diagnosis is often difficult. W...

Seroprevalence of hepatitis C virus among the blood donors in a tertiary care hospital

Background: Transfusion of blood and blood products although considered as a life saving treatment modality, but may lead to certain infectious and non-infectious complications in the recipients. Objectives: The purpose...

Download PDF file
  • EP ID EP419017
  • DOI -
  • Views 136
  • Downloads 0

How To Cite

Shashi Kiran K, Hari Shanker V, Triveni Bhopal, Kandukuri Mahesh Kumar (2016). A study of Bcl-2 and p-53 immunostaining expressions in colonic carcinomas. MRIMS Journal of Health Sciences, 4(1), 28-32. https://europub.co.uk/articles/-A-419017