A STUDY OF DETECTION OF MYCOBACTERIA BY FLUORESCENCE MICROSCOPY IN IMPRINT SMEAR AND ZIEHL-NEELSEN STAIN IN TISSUE SECTION FROM SKIN BIOPSY SPECIMEN
Journal Title: Journal of Evolution of Medical and Dental Sciences - Year 2019, Vol 8, Issue 6
Abstract
BACKGROUND Over the years, a number of methods have been tried to detect the presence of Mycobacteria in tissue samples. A number of previous literatures are available comparing the efficacy of fluorescent microscopy with Ziehl-Neelsen (ZN) stain for the detection of acid-fast bacilli (AFB) in tissue sections, but very few literatures are available on the efficacy of fluorescent method in imprint smears taken from skin biopsy over ZN method in tissue sections. Thus, the present study was undertaken with the following objective- to study the efficacy and advantages of using fluorescent microscopy in imprint smear from skin biopsies over ZN method in tissue sections for detection of mycobacteria in clinically suspected cases of leprosy and cutaneous tuberculosis. MATERIALS AND METHODS 80 patients with a clinical suspicion of leprosy and cutaneous tuberculosis were biopsied & imprint smears taken. Imprint smears were air-dried and stained with Auramine O, and the skin biopsy samples were processed for routine histopathology and ZN Staining. Results were then analysed. RESULTS Out of 80 imprint smears, 65 were studied (remaining 15 were excluded from the study as per exclusion criteria), of which 40% (26/65) were positive for AFB on ZN method, while the smear positivity increased to 49.2% (32/65) on the fluorescent method. CONCLUSION Fluorescent microscopy has the advantage of speed and ease of screening and reduces observer fatigue and found to be more advantageous than conventional ZN method, particularly in paucibacillary and low mycobacterial load cases. Hence, fluorescent method can be an adjuvant when used with routine histopathology for the identification of AFB.
Authors and Affiliations
Priyanka Agarwala, Biswajit Haldar, Rajesh Kr. Mandal, Mohit Agarwal, Aditya Kumar Tewari, Suman Biswas, Subrata Bhattacharjee
Keywords
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- EP ID EP554775
- DOI 10.14260/jemds/2019/79
- Views 61
- Downloads 0
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