A study of holotranscobalamine as sensitive marker in the diagnosis of megaloblastic anemia
Journal Title: MedPulse -International Medical Journal - Year 2017, Vol 4, Issue 3
Abstract
Background: After introduction of the metabolic tests for plasma methylmalonic acid (MMA) and plasma total homocysteine (Hcy), attention has been drawn to vitamin B12 status in asymptomatic individuals. Aims and Objectives: To study holo trans cobalamine as sensitive marker in the diagnosis of megaloblastic anemia. Methodology: After approval from institutionalethical committee this study was carried out at tertiary health care hospital in the 50 patients during January 2016 to December 2016 with clinical features of Megaloblastic anemia The sensitivity, Specificity, Positive Predictive and Negative Predictive Value etc. of HoloTC test with respect to Serum cobalamin test was calculated by Med Cal software. Results: The majority of the Patients were from the age group of 1-10 i.e. 6% followed by 10 -20 - 10 %; in 20-30 was 8%; in 30-40 was 14 %; in 40-50 was 18% and in 50-60 Was 20 % and >60 was 24 %. The majority of the patients were Females i.e. 54% followed by Males i.e. 46. Sensitivity of the Holo TC test is 93.33% with 95 % CI (81.73% to 98.60%) Specificity was 80.00 % (28.36% to 99.49%) and Positive Likelihood Ratio was 4.67 (0.81 to 26.98) and Negative likely hood ratio was 0.08 (0.03 to 0.27) and Positive Predictive Value was 97.67% (87.71% to 99.94%) and Negative Predictive Value was 57.14 % (18.41% to 90.10%). Conclusion: From our study it can be concluded that holotranscobalamine is a sensitive marker with sensitivity of the test 93.33% and Specificity was 80.00 % so this test can be used as screening test us in conjugation with other test like Sr. Cobalamin, plasmamethylmalonic acid (MMA) and plasma total homocysteine (Hcy) etc. for accurate diagnosis of Megaloblastic anemia (Vit B12 Deficiency).
Authors and Affiliations
Kiran A Dawle, Akash Swami
Keywords
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