A study of pectinase enzyme activity changes in gamma- irradiated Trichoderma reesei mutants
Journal Title: International Journal of Farming and Allied Sciences - Year 2014, Vol 3, Issue 5
Abstract
Pectinase enzyme production (due to the many applications in different industries) from fungal sources (such as Trichoderma, which have the potential pathogenicity for humans and animals and microorganisms is considered safe for various industries) due to greater stability and diversity significant in eukaryotes compared to prokaryotes enzyme systems that are currently used for the production of an enzyme compounds have many advantages. Trichoderma reesei is one of the most active isolates in enzyme production that in this study with direct mutation in the genome of the fungus, the activity of this enzyme was investigated in a suitable temperature and pH in order to increase the activity of fungal pectinase. In this case, mutation induction was performed in T. reesei with optimum dose of 250 Gy. After substrate preparation and induction of pectinase, pectinase activity in 21 mutanted isolates were measured. All experiments were conducted in a completely randomized design with three replications. And statistically analyzed using SPSS software and ANOVA and mean comparison using Duncan took a 5% statistical level. Our results showed that, two most active strains were T.r M13 andT.r M 15 with specific activity of the enzyme 4.01U/ml and 2.65 U/ml, on the other hand, isolates T.r M7 and T.r M1 by 0.18 U/ml and 0.16 U/ml specific activity were weakest isolates .In 50% of isolates causing mutations by gamma-ray radiation lead to increased pectinase activity.
Authors and Affiliations
Adeleh sadat Mohamadi, Samira Shahbazi, Mehdi Behgar, Sirous Mansouri fard and Hamed askari
Keywords
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