A study of rdxA gene deletion in metronidazole resistant and sensitive Helicobacter pylori isolates in Kerman, Iran
Journal Title: Jundishapur Journal of Microbiology - Year 2011, Vol 4, Issue 2
Abstract
Introduction and objective: Metronidazole (Mtz) resistance in Helicobacter pylori has been found to be associated with mutations in rdxA, a gene encoding an oxygen insensitive NADPH nitroreductase, and enhanced by mutations in frxA a gene encoding a NAD(P)H-flavin oxidoreductase. In this study, we examined the prevalence of rdxA deletion among local isolates of H. pylori. Materials and methods: We tested 63 H. pylori isolates obtained from 191 patients referred to endoscopy unit of Afzalipour hospital in Kerman, Iran, during 2009 for their susceptibility to common anti-H. pylori antibiotics using a modified disk diffusion test. Then the metronidazole resistant and sensitive isolates were evaluated for deletion in rdxA gene by PCR methods and compared with each other. Results: From 35 resistant H. pylori isolates in this study only 22.9% (8 isolates) detected with deletion rdxA gene. No rdxA deletion was detected in sensitive isolates. Conclusion: According to rdxA deletion rates in this study, it seems that some other nitroreductases are involved in metronidazole activation or there are other metronidazole resistance mechanisms involved.
Authors and Affiliations
Hamid Abdollahi, Mohammad Savari, Mohammad Javad Zahedi, Sodief Darvish Moghadam, Mehdi Hayatbakhsh Abasi
Keywords
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