A study on antibacterial efficacy of different extracts of Artocarpus chama fruits and identification of bioactive compounds in the most potent extract
Journal Title: Jordan Journal of Pharmaceutical Sciences - Year 2022, Vol 15, Issue 1
Abstract
This study is designed to evaluate the antibacterial potential of various extracts of mature green fruits of Artocarpus chama with the identification of bioactive compounds. The antibacterial efficacy was tested against eight pathogenic bacteria using standard antibiotics as the positive control. Antibacterial bioassay was performed by measuring zone of inhibition, Minimum Inhibitory Concentrations (MIC), and Minimum Bactericidal Concentrations (MBC). The most effective extract was further subjected to preliminary phytochemical tests, Thin Layer Chromatography (TLC), and Fourier Transform Infrared analysis. Ethyl acetate extract (EAAC) was found to be the most effective extract producing an inhibition zone of 45.50 ± 0.50 mm against Pseudomonas aeruginosa MTCC 2453. Effective spots (ES) (Rf=0.37) of EAAC separated by TLC analysis were subjected to indirect contact bio-autography for evaluating their efficacies against the tested bacterial strains. Values of MBC/MIC for the extract ranged from 1.20 to 1.80 (<2) indicating their bactericidal property. Gas Chromatography-Mass Spectroscopy analysis of ES was performed for the identification of bioactive antibacterial compounds. Alkaloids, saponins, steroids, terpenoids, and an array of functional groups in EAAC were detected. MICs of ES ranged from 6 - 10 µg/ml. Four bioactive compounds in ES were detected in GC-MS analysis. Thus, this study revealed that ethyl acetate extract of A. chama fruits contained several bioactive compounds with promising bactericidal properties against tested strains of both Gram-positive and Gram-negative pathogenic bacteria.
Authors and Affiliations
Sunanda Burman, Goutam Chandra
Keywords
Related Articles
- EP ID EP711694
- DOI -
- Views 21
- Downloads 0
Molecular modeling studies of some phytoligands from Ficus sycomorus fraction as potential inhibitors of cytochrome CYP6P3 enzyme of Anopheles coluzzii
The major obstacle in controlling malaria is the mosquito’s resistance to insecticides, including pyrethroids. The resistance is mainly due to the over-expression of detoxification enzymes such as cytochromes. Insectici...
Development and Characterization of a Microcrystalline Cellulose-based co-Processed Excipient using Design of Experiment Approach
The study aims to develop a co-processed excipient (CPE) containing microcrystalline cellulose (MCC) and crospovidone (CPV) using the wet massing technique. Preformulation studies were carried out on CPE to characterize...
Antidiabetic activity, polyphenols-based characterization and molecular interaction of extract of un-ripe pods of Vinca rosea cv. Pink
The study aimed to investigate extracts of un-ripe pods of Vinca rosea cv. Pink for antidiabetic activity and polyphenol-based characterization of extracts using HPLC. Moreover, the molecular interaction of the identifi...
Extract and Fractions from Soil Bacteria (Streptomyces canus ATCC 12647) Possess Antimicrobial and Anti-Oxidative Potential in vitro
Streptomyces species are the most prolific producers of antibiotics within the group actinobacteria. The in-vitro antimicrobial and antioxidant activities of the methanol (MeOH) extract and vacuum liquid chromatography...
Chemical Constitution, In-silico Molecular Docking Studies and Antibacterial Activity of Flower Essential Oil of Artabotrys hexapetalus
The isolation of the volatile constituents from the flowers of Artabotrys hexapetalus was carried out using a simple headspace solvent-trapping technique and identified by GC-MS analysis. The major compounds are ethyl a...