A study on antibacterial efficacy of different extracts of Artocarpus chama fruits and identification of bioactive compounds in the most potent extract
Journal Title: Jordan Journal of Pharmaceutical Sciences - Year 2022, Vol 15, Issue 1
Abstract
This study is designed to evaluate the antibacterial potential of various extracts of mature green fruits of Artocarpus chama with the identification of bioactive compounds. The antibacterial efficacy was tested against eight pathogenic bacteria using standard antibiotics as the positive control. Antibacterial bioassay was performed by measuring zone of inhibition, Minimum Inhibitory Concentrations (MIC), and Minimum Bactericidal Concentrations (MBC). The most effective extract was further subjected to preliminary phytochemical tests, Thin Layer Chromatography (TLC), and Fourier Transform Infrared analysis. Ethyl acetate extract (EAAC) was found to be the most effective extract producing an inhibition zone of 45.50 ± 0.50 mm against Pseudomonas aeruginosa MTCC 2453. Effective spots (ES) (Rf=0.37) of EAAC separated by TLC analysis were subjected to indirect contact bio-autography for evaluating their efficacies against the tested bacterial strains. Values of MBC/MIC for the extract ranged from 1.20 to 1.80 (<2) indicating their bactericidal property. Gas Chromatography-Mass Spectroscopy analysis of ES was performed for the identification of bioactive antibacterial compounds. Alkaloids, saponins, steroids, terpenoids, and an array of functional groups in EAAC were detected. MICs of ES ranged from 6 - 10 µg/ml. Four bioactive compounds in ES were detected in GC-MS analysis. Thus, this study revealed that ethyl acetate extract of A. chama fruits contained several bioactive compounds with promising bactericidal properties against tested strains of both Gram-positive and Gram-negative pathogenic bacteria.
Authors and Affiliations
Sunanda Burman, Goutam Chandra
Keywords
Related Articles
- EP ID EP711694
- DOI -
- Views 25
- Downloads 0
Morphological Variability Assessment of Worldwide Germplasm of Pharmaceutically Important Plant Nigella Sativa L.
N. sativa is an important industrial crop globally but neglected crop in Pakistan, hence imported. It has been used for revitalizing body system in almost all ancient civilizations. In the present investigation worldwid...
Chemical and Biological Investigation of Sanchezia nobilis Leaves Extract
The project work was designed to investigate the phytochemical and selected pharmacological activities (anti-diarrheal, analgesic, neuropharmacological behavior and anthelmintic) of leaves of Sanchezia nobilis. Hook. F....
Knowledge, Attitudes and Practice toward Antibiotic Use among Under and Post-Graduate Students at Yarmouk University in Jordan: A Descriptive Study
Objective: Irrational and overzealous use of antibiotics in addition to multidrug resistance has increased at an alarming limits around the world. This study aimed to evaluate the knowledge and practices toward antibio...
Extract and Fractions from Soil Bacteria (Streptomyces canus ATCC 12647) Possess Antimicrobial and Anti-Oxidative Potential in vitro
Streptomyces species are the most prolific producers of antibiotics within the group actinobacteria. The in-vitro antimicrobial and antioxidant activities of the methanol (MeOH) extract and vacuum liquid chromatography...
Evaluation of Basella alba L. Mucilage as a Suspending Agent in Metronidazole Suspension
In the quest for natural biodegradable, non-toxic polymers for use as excipients in pharmaceutical formulations, mucilage of Basella alba L (BAM) stem was isolated and evaluated as a suspending agent in metronidazole su...