A study on carbapenemase producing Acinetobacter sp., and identification of OXA-51 gene in isolates from patients attending a tertiary care hospital
Journal Title: Indian Journal of Microbiology Research - Year 2018, Vol 5, Issue 1
Abstract
Introduction Acinetobacter sp are Gramnegative nonfermenters that causes nosocomial infections Multi drug resistance has been evolving very rapidly in Acinetobacter sp with also a dramatic increase in carbapenem resistance Even though there are various mechanisms prevailing for such resistance detection of Class D beta lactamases oxacillinases production is cumbersome Accurate detection is done only by genotypic methodsAims and objectives In order to estimate the prevalence of Acinetobacter sp its antimicrobial susceptibility pattern the following study was conducted in a tertiary care hospital Organisms resistant to Carbapenems were further tested for OXA51 gene identification by polymerase chain reactionMaterials and Methods All the study samples received during the study period were subjected to microbiological culture method and identification panel to isolate Acinetobacter sp Antimicrobial susceptibility testing was done according to CLSI guidelines Organisms that fit the criteria of multidrug resistant were further analysed for Carbapenem resistance by disc diffusion test and those isolates were subjected to polymerase chain reaction for identifying the presence of OXA51 geneResults A total of 102 48 Acinetobacter sp isolates were obtained from a total sample size of 2127 specimens during the study period Over all 6078 of isolates were multidrug resistant MDR and 5098 were imipenam Carbapenem resistant All the imipenam resistant isolates were belonging to Abaumanii A total of 52 isolates subjected to PCR showed positivity for OXA51 geneConclusion This warrants the need for genotyping for all of the Acinetobacter isolates to understand the gene variations in resistance and to know whether there is a universal presence of OXA51 gene in all of the Acinetobacter baumannii
Authors and Affiliations
Pradha Velu, Ramya Rengaraj
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