A study on determination of Minimum Inhibitory Concentration (MIC) of Vancomycin of MRSA Isolates and their impact in treatment of MRSA Isolates
Journal Title: IP International Journal of Medical Microbiology and Tropical Diseases - Year 2017, Vol 3, Issue 1
Abstract
Introduction: Staphylococcus aureus is one of the most common causes of nosocomial infections. Methicillin-resistant S. aureus (MRSA) is among the top three clinically important pathogens. The glycopeptide vancomycin is considered to be the best alternative for the treatment of MRSA. MRSA usually exhibit vancomycin-susceptible phenotype (VSSA) but some strains exhibit reduced susceptibility to vancomycin which can be heterogeneous-intermediate (hVISA), intermediate (VISA) or fully resistant (VRSA) phenotypes which results in treatment failure. More recently, poor clinical outcome is observed in infections with MRSA strains with an elevated levels of vancomycin MIC within the susceptible range. Aim: This study was done to know the prevalence of MRSA and to determine the vancomycin MIC. Materials & Methods: S.aureus isolated from clinical samples were screened for methicillin resistance using cefoxitin discs (30 μg). The vancomycin MIC of these MRSA isolates was determined using E-strips. Results & Discussion: A total of 102 isolates of S.aureus were subjected to study. Among these, 42 isolates were MRSA (41.2%). The different MIC values are as follows: 0.38 μg/mL (2 isolate), 0.75 μg/mL (1 isolate), 1 μg/mL (3 isolates), 1.5 μg/mL (32 isolates) & 2 μg/mL (4 isolates). Although all the MRSA strains were within the susceptible range of vancomycin MIC, their increased MIC values (>1 μg/mL) can lead to treatment failures. Conclusion: Increased risks of treatment failure has been observed in infections caused by MRSA isolates with vancomycin MIC in the upper end of susceptible range (MIC > 1µg/ml), emphasising the need for determination of vancomycin MIC to assess the treatment outcome.
Authors and Affiliations
Mathavi Suresh Kumar, Vijai Radhika, Kavitha A, Sasikala G, Indra Priyadharsini
Keywords
Related Articles
- EP ID EP305097
- DOI 10.18231/2455-6807.2017.0004
- Views 131
- Downloads 0
Detection of gyrA ser83 mutation and parC gene amplification in E.coli and Klebsiella pneumoniae from tertiary care hospital, Puducherry
Introduction: Fluoroquinolones are the broad spectrum antibiotics and recently the clinical isolates of Enterobacteriaceae species had slowly extend the high resistance towards them. The point mutation in gyrA gene had s...
A study of intestinal parasitic infections in HIV infected individuals in Puducherry
Introduction Human immuno deficiency virus infection has become a pandemic Infection with HIV results in a chronic illness characterized by a progressive decline in cellmediated immune function Diarrhoea occurs in almost...
Bacteriological profiles in burn patients within first twenty-four hours of injury
Background: Infection and sepsis are the main confounders of mortality and morbidity of burn injury. Burn wound surface thought to be sterile for few hours immediately after injury. Eventually microorganisms from skin su...
Corynebacterium tuberculostearicum a potential pathogen in breast abscess- A case report
Gram positive aerobic rods belonging to the genus Corynebacterium colonize the skin and mucosal surfaces of humans They are frequently isolated from various clinical specimens Interpretation of their clinical relevance i...
Detection of efflux pump in multidrug resistant acinetobacter Species using Naphthyl-Methyl-Piperazine
Acinetobacter Gram negative bacterium has proven to be recently important to research due to multidrug resistance Multidrug resistance is directed against antibiotics like Aminoglycosides Carbapenems Cephalosporins Quino...