A Study on Endogenous Hormone Levels and Phenolic Profiles in Embryogenic and Non-Embryogenic Calli of Endemic Plant Campanula tomentosa L.
Journal Title: Asian Journal of Biology - Year 2017, Vol 4, Issue 2
Abstract
A micropropagation method was developed for the first time in Campanula tomentosa L. (Campanulaceae), adapted to chasmophytic habitats and colonized on calcareous rocky cliffs. Seeds of C. tomentosa were germinated on ½ MS(33%), N6 (30%), B5(11%) and MS(4%) media for callus initiation, leaf explants taken from sterile seedlings were cultured after they were transferred to the MS, ½ MS, N6 and B5 media. The effect of the auxin/cytokine ratio in media and the quality of the callus varied depending on the nutrient medium made formation of a different callus type. The development of two callus types was observed, type I composed of globular embryos was observed in the MS medium (85.71%) and type II translucent, watery and lacking any sign of organization was observed in the B5 medium (71.43%). The objective of this study was to investigate how acetone solvent systems affected the culture media of the total phenolic content (TPC), and condensed tannins content (CTC) in embryogenic, non-embryogenic callus and in vitro germinated roots. The results showed that the 50% acetone extracts exhibited the highest TPC and CTC for both1/2 MS type II. The 80% acetone extracts exhibited the highest TPC for N6 root and CTC for 1/2 MS root.
Authors and Affiliations
Melek Işık Coşkun, Meliha Gemici, Hasan Yildirim
Keywords
Related Articles
- EP ID EP337791
- DOI 10.9734/AJOB/2017/34635
- Views 51
- Downloads 0
Phytochemical Analysis and Nematicidal Activity of Ethanolic Leaf Extracts of Datura metel, Datura innoxia and Brugmansia suaveolens Against Meloidogyne incognita
The present study was aimed to screen the phytochemicals and quantification of alkaloids, phenolic compounds and flavonoids and to evaluate the nematicidal activity of ethanolic leaf extracts of Datura metel, Datura inno...
Prevalence of Entamoeba histolytica Infection Using Microscopy and Adhesin Detection Methods among School Children in Central Nigeria
This study aimed to evaluate the prevalence of Entamoeba histolytica infection using microscopy and adhesin detection methods among school children in Central Nigeria. After ethical clearance, stool samples of three hund...
Antioxidant and Anticancer Activities of Extracts and Compounds Isolated from Terminalia nigrovenulosa Plant Grown in Vietnam
This study was to isolate and identify antioxidant and anticancer compounds from extracts of bark and leaf of Terminalia nigrovenulosa. The EtOAc fraction of bark and n-BuOH fraction of leaf exhibited the highest DPPH (2...
Evaluation of Botanicals Toxicants against Root-knot Nematode, Meloidogyne incognita in vitro
The present experiment was carried out to investigate the nematostatic and nematicidal potential of aqueous extracts of six weed leaves viz., Ageratum conyzoides, Eichornia crassipes, Ipomoea carnea, Nicotiana plumbagini...
An Overview of Nickel (Ni2+) Essentiality, Toxicity and Tolerance Strategies in Plants
Heavy metals (HMs) toxicity has an unavoidable threat to environment and public health due to their increasing contamination and accumulation in atmosphere which ultimately passes to the living beings by the route of foo...