A study on Methicillin resistant Staphylococcus aureus mastitis in dairy cows
Journal Title: Journal of Applied and Natural Science - Year 2014, Vol 6, Issue 2
Abstract
Methicillin-resistant Staphylococcus aureus (MRSA) poses a serious problem in dairy animals suffering from mastitis. The study was carried out to evaluate the incidence of Methicillin resistant S. aureus from clinical mastitis milk samples and their antibiotic resistance profile and characterised with respect to the molecular features that contributed to the resistance in these pathogens. Isolation and identification of Methicillin resistant S. aureus were performed from acute clinical mastitis samples. The isolates were tested using agar disc diffusion method for their antimicrobial susceptibility and modified resazurin assay micro dilution technique for MIC to 8 different antimicrobial drugs. A total of 235 clinical mastitis milk samples from dairy cows were cultured for incidence of S. aureus. Methicillin resistant S. aureus was isolated from a total of 12 (44.25%) of the 116 S. aureus samples. Based on the antimicrobial sensitivity and MIC results, MRSA isolates were found sensitive to gentamicin, enrofloxcain, amoxicillin+sulbactam, ceftriaxone and resistant to amoxicillin, oxytetracycline, penicillin G and oxacillin. Most of MRSA isolates were found to be multi-drug resistant. MRSA alert kit test and mecA and blaZ target gene PCR were found to be useful in the confirmation of MRSA
Authors and Affiliations
D. Chandrasekaran, P. Venkatesan, K. Tirumurugaan, A. Nambi, S. Vairamuthu
Keywords
Related Articles
- EP ID EP158255
- DOI 10.31018/jans.v6i2.427
- Views 113
- Downloads 0
Effect of weed management on yield and nutrient uptake in mustard (Brassica juncea)
Field investigation was carried out during rabi season of 2014-15 at Udaipur to evaluate the effect of different weed management practices on yield and nutrient uptake of mustard. The maximum seed yield was registered wi...
Enhancing seedling production of black velvet tamarind (Dialium guineense Willd) 1
The present investigation was conducted to enhance seedling production of Black Velvet Tamarind (Dialium guineense). Three different types (sets) of experiments were designed. The results showed no sprouting of the stem...
Status and socio-ecological dimensions of sacred groves in Northeast India
Sacred groves are among one of the best practices of biodiversity conservation used by the ethnic societies which are deeply associated with the religion and culture that significantly nurture nature. As these practices...
Variability for seed oil content and seedling traits in Pongamia pinnata (L.) Pierre
Twenty three CPTs (Candidate Plus Trees) of Pongamia pinnata were selected from different agro-climatic conditions of Haryana state of India and were assessed to identify the elite planting material for improvement of th...
Management of purple blotch and Stemphylium blight of onion in Tarai and Bhabar regions of Uttarakhand, India
Purple blotch [Alternaria porri] (Ellis) Cif.) and Stemphylium blight [Stemphylium vesicarium] (Wallr.) E. Simmons) of onion cause serious problems in onion seed crop in Tarai and Bhabar region of Uttarakhand. In present...