A Study on Prevalence and Antibiotic Susceptibility Pattern of Vancomycin Intermediate and Resistant Staphylococcus Aureus in Clinical Specimen in a Tertiary Care Hospital and Detection of their MIC Values by E-test.
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2018, Vol 17, Issue 12
Abstract
With the increasing incidence of Methicillin Resistant Staphylococcus Aureus(MRSA), Vancomycin Intermediate Staphylococcus Aureus(VISA) & Vancomycin Resistant Staphylococcus Aureus (VRSA) strains now a days. The study was conducted to find out the magnitude of vancomycin resistance in both MRSA and MSSA and antibiotic susceptibility pattern of those isolates in a tertiary care hospital, Jaipur between January 2017 to June 2018.In this cross sectional study,287 Staphylococcus aureus were isolated and identified from various clinical specimens collected from different departments of the hospital.Among these 287 strains,146 were found to be MRSA and 141 MSSA. Subsequently ,the antimicrobial susceptibility test was performed by Kirby Bauer disc diffusion method as per CLSI guidelines. All strains found resistant to vancomycin by disc diffusion method were again grown on BHI-VSA(Brain heart infusion-Vancomycin screen agar) and also recruited to E- Test for confirmation of resistance. Minimum Inhibitory Concentration (MIC) of ≤2 were considered as VSSA,4-8 as VISA.MIC of ≥16 as VRSA. Vancomycin resistance was seen in 9 isolates of S. aureus by disc diffusion method. Among them neither was found to be VISA nor VRSA when confirmed with ETest with all having MIC <2 .Though there was no incidence of VRSA but MIC of 1.5µg/ml in our study rang an alarm to the infection control committee of this tertiary care hospital.
Authors and Affiliations
Dr. Prassana Gupta, Dr. Rahul Bhargava
Keywords
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- EP ID EP423829
- DOI 10.9790/0853-1712052127.
- Views 62
- Downloads 0
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