A study on prevalence of Methicillin and Vancomycin resistance among Staphylococcus aureaus isolates in an Iranian 1000-bed tertiary Care Hospital
Journal Title: International Journal of Medical Research & Health Sciences (IJMRHS) - Year 2016, Vol 5, Issue 3
Abstract
Methicillin resistant Staphylococcus aureus (MRSA) is one of the most important etiology agent of community and hospital acquired infections. In our country, the prevalence of MRSA is estimated to be 50%. The aim of present study was to determine the prevalence of MRSA and Vancomycin resistant Staphylococcus aureus isolates in an Iranian 1000-bed tertiary care hospital. During a period of September 2013 and June of 2014 a total of 220 nonduplicate S.aureus strains were isolated from clinical specimens. PCR and Cefoxitin disk diffusion methods were used for detection of MRSA. Vancomycin screen agar (BHI agar containing 6µ/ml Vancomycin) and E-test MIC method were used for detection of VISA VRSA. The susceptibility testing of isolates to other antibiotics was used by the disk diffusion method as recommended by CLSI. In our study 145 (65.90%) out of 220 isolates were isolated from urine and the others were isolated from other specimens such as wound and tracheal aspirates. By PCR method 105(47.72%) isolates were found to be MRSA. In our study the results of Cefoxitin disk diffusion method was in accordance to PCR. No VISA and VRSA isolates was determined by Vancomycin screenagar and E-test. The sensitivity and specificity of Cefxoitin disk diffusion method for detection of MRSA was 100%.It is concluded that the rate of MRSA and VRSA in our study was 47.72% and 0% respectively. In laboratories with limited sources for performance PCR, other methods such as Cefoxitin disk diffusion and Vancomycin screen agar are the best alternative methods for detection of MRSA, VISA and VRSA.
Authors and Affiliations
Hossein Koupahi| Department of Microbiology, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran, Sahar Honarmand Jahromy| Department of Microbiology, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran, Mohammad Rahbar| Department of Microbiology, Iranian Reference Health Laboratory Research Center, Ministry of Health and Medical Education, Tehran, Iran, Corresponding Email: rahbar.reflab@gmail.com, Parisa Eslami| Department of Microbiology, Central laboratory, Milad Hospital, Tehran Iran, Ehsan Khodadadi| Department of Molecular Genetics and Genetic Engineering, Tabriz Branch, Islamic Azad University, Tabriz, Iran
Keywords
Related Articles
- EP ID EP11613
- DOI -
- Views 262
- Downloads 10
Comparative Analysis of Region of Interest of Different Sizes for Breast Density Classifcation
In medical feld, radiologists are more interested in region of interest (ROI) rather than whole image. ROI is a subpart of the image that contains very important information related to the diagnosis. In addition, ROI si...
A Rare Case Report of Subcutaneous Mycoses by Rhytidhysteron Rufulum
Phaeohyphomycosis is a term to describe cutaneous and systemic or disseminated mycoses caused by a variety of dematiaceous fungi. Rhytidhysteron rufulum is a poorly known, common, pantropical phaeoid fungal species, and...
The Effect of Glycine Treatment on Histomorphometric Changes in the Diabetic Rat Retina
iabetic retinopathy is a major cause of blindness and one of its possible mechanisms is impairment in proteins and amino acids synthesis due to impairment in glucose synthesis. So far, different amino acids have been us...
Incidence of Radial Artery Occlusion After One Year in Patients Underwent Radial Interventions with Preprocedural Patent Radial Artery by Barbeau Test and Its Comparison with the Doppler Examination
Aim of the study: To fnd the incidence of radial artery occlusion (RAO) at one year in patients who have radial interventions by using the Barbeau test. Materials and methods: Totally 105 patients are selected. Prior to...
Reviewing the Relation between Social Support and Blood Sugar Control in Elders with Diabetes Type 2
Since a major part of curing the elders' diabetes occurs at home, family and relatives play an important role in keeping these patients. This study aims to review the relation between social support and blood sugar cont...