A Study on the Evaluation of Typhidot M (IgM Enzyme-linked Immunosorbent Assay) in the Early Diagnosis of Enteric Fever in Children
Journal Title: INTERNATIONAL JOURNAL OF SCIENTIFIC STUDY - Year 2018, Vol 6, Issue 4
Abstract
Introduction: Enteric fever, or typhoid, is a major public health problem in developing countries like India. The causative organisms, Salmonella typhi and paratyphi, are developing multidrug resistance due to indiscriminate use of antibiotics even in undeserving cases. Thus, enteric fever needs to be diagnosed early not only to reduce morbidity but also to combat antimicrobial resistance. Aims and Objectives: This study aims to determine the utility of Typhidot M (IgM enzyme-linked immunosorbent assay [ELISA]) in the early diagnosis of enteric fever in children and to determine its sensitivity, specificity, and positive and negative predictive value in comparison with Widal, keeping blood culture as the gold standard. Materials and Methods: Children from 1 to 12 years (n = 90) with clinical suspicion of enteric fever based on a set of criteria were enrolled in the study. Blood for blood culture, Typhidot M, and Widal were drawn, and the results analyzed. Results: Among 90 children enrolled, 11 (12.22%) had positive cultures. 24 (26.67%) had positive Widal tests while 58 (64.44%) were positive for IgM antibodies by Typhidot M test. The sensitivity, specificity, and positive and negative predictive values of Typhidot M test were found to be 100%, 40.5%, 18.96%, and 100%, respectively, while that for Widal were 90.91%, 82.28% 41.66%, and 98.45%, respectively. Conclusion: Typhidot M (IgM ELISA) is a simple, sensitive, and reliable test for the early diagnosis of typhoid fever in children.
Authors and Affiliations
P Murugalatha
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