A three-dimensional analysis of jejunal myenteric ganglia: a tool for interpretation of the optical recording

Journal Title: Archives of Medical Science - Year 2006, Vol 2, Issue 4

Abstract

Introduction: Investigating functions of neurons in the enteric nervous system using imaging techniques (e.g. voltage sensitive dyes) requires precise data concerning the three-dimensional structure of enteric ganglia. Recording from small enteric ganglia such as the submucosal plexus allows for single cell resolution recording, while myenteric ganglia are more complex and single cell resolution claim is not straightforward. Therefore, the aim of the present study was to analyze appositions of neuronal somata in myenteric ganglia. Material and methods: Stretched samples of guinea pig jejunum were fixed and dissected to expose the myenteric plexus. Tissues were stained for Hu protein and confocal microscopic analysis of myenteric ganglia was performed in order to reconstruct three-dimensional allocations of neuronal somata within the myenteric plexuses. Results: Myenteric neurons form a three-dimensional structure. Analysis of 103 cells showed that 20% were not overlapping with other cells, while 30% and 31% overlapped with 1 and 2 cells, respectively. Looking at the area of overlap, 42% of cells shared less than 10% of their area. On the other hand, 17% of cells had more than 50% of their area shared with others. Conclusions: These results show that in guinea pig myenteric ganglia neuronal somata form a monolayer, but up to half of the cells may have a significant overlap with other cell somata. Therefore all optical studies claiming single cell resolution recorded from the myenteric plexus should be confirmed by confocal analysis of the ganglia using immunohistochemical identification of cellular structure of analyzed plexuses.

Authors and Affiliations

Michał Ceregrzyn, Jarosław Woliński, Birgit Kuch

Keywords

Related Articles

Clinical Study and Management of Secondary Peritonitis due to Perforated Hollow Viscous

Introduction: Secondary peritonitis is an inflammation of the peritoneum caused by another condition, most commonly the spread of an infection from the digestive organs or bowels. This paper aims to present a clinical st...

Pregnancy and delivery in women with congenital heart disease after cardiac surgery

Introduction: Women with congenital heart disease (CHD) are the majority of pregnant cardiac patients. Pregnancy and delivery care in these patients is still an important clinical issue. The aim of the study was to an...

Sexual dysfunctions and marital adjustment in veterans with PTSD

Introduction: Considering that we have in Iran a lot of veterans who suffer from post-traumatic stress disorder (PTSD), this research was performed to identify sexual dysfunction among a group of veterans who suffer from...

Download PDF file
  • EP ID EP91202
  • DOI -
  • Views 162
  • Downloads 0

How To Cite

Michał Ceregrzyn, Jarosław Woliński, Birgit Kuch (2006). A three-dimensional analysis of jejunal myenteric ganglia: a tool for interpretation of the optical recording. Archives of Medical Science, 2(4), 226-234. https://europub.co.uk/articles/-A-91202