A Validated Chiral HPLC Method For Enantiomeric Separation of Glycidyl Butyrate on Cellulose Based Stationary Phase
Journal Title: IOSR Journal of Applied Chemistry (IOSR-JAC) - Year 2018, Vol 11, Issue 9
Abstract
A Chiral high-performance liquid chromatographic (HPLC) method was developed and validated for the enantiomeric separation of glycidyl butyrate and the quantification of S (+)-glycidyl butyrate. Separation was achieved on Chiralcel OD-H (250 × 4.6mm, 5µ) column using a mixture of Hexane: 2-Propanol (100:1, v/v) as a mobile phase with flow rate of 1.0 mL/min on UV detector. The resolution between S (+)-glycidyl butyrate (SGB) and R (-)-glycidyl butyrate (RGB) enantiomers was found to be more than 2. The proposed method was validated with respect to linearity, precession, accuracy, limit of detection (LOD), limit of quantitation (LOQ) and robustness. The SGB showed an excellent linearity over the concentrations range from 2 to 24 µg/mL, while the mean recovery of SGB was found to be 99.26%. The proposed method was found simple, precise, accurate and robust for quantitative determination of SGB in RGB. The developed method possess advantages such as shorter run time, better resolution between enantiomers, rapid analysis with less solvent consumption, which makes the method economical and environment friendly.
Authors and Affiliations
Udaykumar Rakibe, Ravindra Yeole, Vipul Rane, Prashant Musmade, Ravi Tiwari, Pravin Wakte
Keywords
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- EP ID EP414392
- DOI 10.9790/5736-1109011218.
- Views 150
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