A VALIDATED ISOCRATIC RP-HPLC METHOD FOR CONCURRENT ESTIMATION OF GYMNEMAGENIN, GALLIC ACID AND 18Î’-GLYCYRRHETINIC ACID IN POLYHERBAL FORMULATION
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2014, Vol 6, Issue 8
Abstract
Objective: To develop and validate a simple, precise, selective, and accurate reversed phase high performance liquid chromatography method for concurrent analysis of gymnemagenin, gallic acid and 18β-glycyrrhetinic acid in polyherbal formulation.Methods: The chromatographic separation was achieved on a Thermo Synchronis C18, 5 μm, 250 × 4.6 mm i. d. analytical column. The mobile phase comprised of methanol: water (88: 12, v/v), pH 3.1 adjusted with orthophosphoric acid. The flow rate was kept at 0.8 mL min-1. Quantitation was achieved with UV detection at 218 nm, based on peak area.Results: The retention time for gallic acid, gymnemagenin, and 18β-glycyrrhetinic acid was found to be 3.08, 4.15, and 10.30 min, respectively. Validation of the RP-HPLC method was performed as per International Conference on Harmonization (ICH) Q2 (R1) guideline. The proposed method showed good linearity in the range of 100-1000 μg mL-1 for gymnemagenin, 2.5-50 μg mL-1 for gallic acid and 50-500 μg mL-1 for 18β-glycyrrhetinic acid. The % content of gymnemagenin, gallic acid and 18β-glycyrrhetinic acid in the marketed formulation was found to be 0.1320, 0.2129 and 0.2799 %, respectively.Conclusion: The proposed method can be useful in the quality control of gymnemagenin, gallic acid and 18β-glycyrrhetinic acid in polyherbal formulation.
Authors and Affiliations
Sachin Eknath Potawale, Pravin Devidas Pawar, Satish Yashwant Gabhe, Kakasaheb Ramoo Mahadik
Keywords
Related Articles
- EP ID EP579759
- DOI -
- Views 62
- Downloads 0
2.4 GHZ HETERODYNE RECEIVER FOR HEALTHCARE APPLICATION
The objective of this research was to design a basic 2.4 GHz heterodyne receiver for healthcare on a 130um CMOS process. The ultimate goal for the wireless industry is to minimize the trade-offs between performance and c...
THE STUDY OF THE ACUTE AND SUB-ACUTE ORAL TOXICITIES OF THE NEBULIZED EXTRACT OF MYRACRODRUON URUNDEUVA ALLEMÃO IN RABBITS
Objective: The aim of this research was to evaluate the acute and sub-acute oral toxicities of the nebulized dried extract of Myracrodruon urundeuva (NDEMU) leaf obtained by the spray drying technique on rabbits.Methods:...
A GLOBAL COMPARISON AND ANALYSIS OF NEURAMINIDASE H1N1 STRAIN OF INFLUENZA A
Objective: To evaluate the variation of neuraminidase (NA) protein from various H1N1 strain for drug designing.Methods: In this study we have used 12 sequences of NA protein from various countries, retrieved from Uniprot...
MOLECULAR DOCKING STUDY OF SIX PYRIMIDINE DERIVATIVES AS EGFR (EPIDERMAL GROWTH FACTOR RECEPTOR) AND CA IX (CARBONIC ANHYDRASE IX) INHIBITOR
Objective: The present study was carried out to discover whether these pyrimidine derivatives have the potential to be used as epidermal growth factor receptor (EGFR) and carbonic anhydrase (CA) IX inhibitors through str...
ARTHROBACTER AS BIOFACTORY OF THERAPEUTIC ENZYMES
Therapeutic enzymes are proteins which can be used to treat rare and deadly diseases. They represent a small but profitable market. Therapeutic enzymes are superior to non-enzymatic drugs owing to their high specificity...