Abortifacient Potential Effect of Aqueous Extract of Millittiaaboensis on Reproductive Health of Matured Wistar Rats
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2016, Vol 11, Issue 6
Abstract
The present study evaluated the abortifacient potential of aqueous extract of Millittiaaboensis on reproductive health of matured Wistar rats by assessing some fertility indices, hormonal profile and litter size of pregnant wistar rats. A total of thirty (30) matured female wistar ratswere used for this study. Each matured female rat was mated with two male rats (2:1) until pregnancy was established using vaginal smear method and cage side observations. The pregnant rats were randomized into six (6) groups with five (5) animals in each group. Group 1 animals served as control group and were not treated with the extract but provided with food and water ad libitum. Groups 2,3,4,5 and 6 received concentrations of 1000,2000,3000,4000 and 5000mg/kg body weight of the extract accordingly. All animals were allowed to go full term and sacrificed thereafter. The foetuseswere observed for signs of clinical toxicity, counted and weighed. Blood was collected for hormonal assay. Clinical toxicity such as respiratory distress, salivation, changes in appearance of hair were not observed during the exposure period in the extract treated animals. However, maternal mortality was observed upon administration of 4000 and 5000mg/kg body weight of the extract. Diarrhoea (evident from watery stool) and fatigue was also observed. The final weights of the rats increased significantly (p<0.05) when compared with the initial weights. None of the rats showed signs of vaginal bleeding or expulsion of the products of conception. With these notwithstanding, the weights of the foetuses decreased significantly (p<0.05) in the groups administered 4000 and 5000mg/kg body weight of the extract. The litter size and number of pups at birth also showed a decrease when compared with the control group. The durationof pregnancy was shortened to 20 days in animals that received the extracts at 3000-5000mg/kg body weight. No morphological abnormality was observed in the pups. The crown-rump length also decreased in the treated groups. The Results ofthe female reproductive hormones of the treated animals revealed that FSH, LH and prolactin were significantly reduced (p<0.05) in all the treated groups by the extract. Progesterone and oestrogen were also reduced significantly at 4000 and 5000mg/kg body weight of the extract.
Authors and Affiliations
B. M. Onyegeme- Okerenta1 ,, F. C. Anacletus, K. Offor2
Keywords
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- EP ID EP386751
- DOI 10.9790/3008-1106021319
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