Acetylcholinesterase, butyrylcholinesterase and paraoxonase 1 activities in rats treated with cannabis, tramadol or both
Journal Title: Asian Pacific Journal of Tropical Medicine - Year 2016, Vol 9, Issue 11
Abstract
Objective: To investigate the effect of Cannabis sativa resin and/or tramadol, two commonly drugs of abuse on acetylcholinesterase and butyrylcholinesterase activities as a possible cholinergic biomarkers of neurotoxicity induced by these agents. Methods: Rats were treated with cannabis resin (5, 10 or 20 mg/kg) (equivalent to the active constituent D9-tetrahydrocannabinol), tramadol (5, 10 and 20 mg/kg) or tramadol (10 mg/kg) combined with cannabis resin (5, 10 and 20 mg/kg) subcutaneously daily for 6 weeks. Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) activities were measured in brain and serum. We also measured the activity of paraoxonase-1 (PON1) in serum of rats treated with these agents. Results: (i) AChE activity in brain increased after 10–20 mg/kg cannabis resin (by 16.3– 36.5%). AChE activity in brain did not change after treatment with 5–20 mg/kg tramadol. The administration of both cannabis resin (5, 10 or 20 mg/kg) and tramadol (10 mg/kg) resulted in decreased brain AChE activity by 14.1%, 12.9% and 13.6%, respectively; (ii) BChE activity in serum was markedly and dose-dependently inhibited by cannabis resin (by 60.9–76.9%). BChE activity also decreased by 17.6–36.5% by 10–20 mg/kg tramadol and by 57.2–63.9% by the cannabis resin/tramadol combined treatment; (iii) Cannabis resin at doses of 20 mg/kg increased serum PON1 activity by 25.7%. In contrast, tramadol given at 5, 10 and 20 mg/kg resulted in a dose-dependent decrease in serum PON1 activity by 19%, 36.7%, and 46.1%, respectively. Meanwhile, treatment with cannabis resin plus tramadol resulted in 40.2%, 35.8%, 30.7% inhibition of PON1 activity compared to the saline group. Conclusions: These data suggest that cannabis resin exerts different effects on AChE and BChE activities which could contribute to the memory problems and the decline in cognitive function in chronic users.
Authors and Affiliations
Omar M. E. Abdel-Salam
Keywords
Related Articles
- EP ID EP281736
- DOI 10.1016/j.apjtm.2016.09.009
- Views 104
- Downloads 0
A study on the optimization and characteristics of enzymatic preparation of Artocarpus heterophyllus Lam (Jackfruit) seed resistant starch
Objective: With the development of the Artocarpus heterophyllus Lam (A. heterophyllus) processing industry, A. heterophyllus Lam seed as by-products, usually are cooked to eat or discarded, and no industrial application...
Dengue outbreaks in Taiwan, 1998-2017: Importation, serotype and temporal pattern
Objective: To ascertain the role of imported cases and serotypes on dengue outbreaks in Taiwan which have been sporadic yet highly volatile during the past two decades, exhibiting record-breaking magnitude in recent year...
Nested multiplex PCR for identification and detection of human Plasmodium species including Plasmodium knowlesi
Objective: To develop a new technique for diagnosis of Plasmodium knowlesi and at the same time to be able to discriminate among the diverse species of Plasmodium causing human malaria. Methods: In this study the nested...
Annona muricata: Is the natural therapy to most disease conditions including cancer growing in our backyard? A systematic review of its research history and future prospects
Annona muricata (A. muricata) is a tropical plant species belonging to family Annonaceae and known for its many medicinal uses. This review focuses on the research history of its traditional uses, phytochemicals, pharmac...
Meteorological influences on dengue transmission in Pakistan
Objective: To identify the influences of local and regional climate phenomena on dengue transmission in Lahore District of Pakistan, from 2006 to 2014. Methods: Time-series models were applied to analyze associations bet...