Activity and stability of immobilized Candida rugosa lipase on chitosan coated Fe3O4 nanoparticles in aqueous and organic media
Journal Title: JOURNAL OF ADVANCES IN CHEMISTRY - Year 2014, Vol 10, Issue 3
Abstract
Fe3O4 (magnetite) nanoparticles were prepared by coprecipitation method, coated by chitosan and functionalized by glutaraldehyde. Lipase enzyme from Candida rugosa was immobilized on the prepared particles via cross linking reaction. Synthesis steps and characterization were examined by XRD, TEM, and FTIR. The immobilization conditions were 10 mL of phosphate buffer (0.1 M, pH 6.5) containing 30 mg of functionalized magnetic chitosan nanoparticles and 2.0 mg·mL-1 of lipase, immobilization temperature of 4 ℃ and immobilization time of 1 h. Under these conditions, lipase was successfully immobilized with loading capacity of 87 mg/g. The immobilized enzyme showed good operational and storage stability, where it remained stable after 30 days of storage at 4◦C.and retained about 61% of its initial activity after twenty repeated uses. Finally enzymatic catalyze synthesis of butyl and hexyl oleate at 40 ◦C with shaking (200 rpm) was realized in n-hexane and confirmed by GC analysis.
Authors and Affiliations
Mohamed A. Abd-Elhakeem, Ahmed M. Elsayed, Taher A. Alkhulaqi
Keywords
Related Articles
- EP ID EP652744
- DOI 10.24297/jac.v10i3.6652
- Views 151
- Downloads 0
COMPLEXATION OF POLYETHYLENE-GLYCOL WITH THE SODIUM SALTS OF CITRIC AND SUCCINIC ACIDS IN THE AQUEOUS SOLUTIONS. STUDIES BY DYNAMIC LIGHT SCATTERING AND UV/VIS SPECTROPHOTOMETRY
Dynamic light scattering (DLS) method in combination with the UV/VIS spectrophotometry is used to study the interaction of polyethylene- glycols with a molecular weight 6000 ( PEG6000 ) with sodium salts of citric and...
Gas Chromatography-Mass Spectrometer Electron Ionization (GC-MS -EI)method for the Analysis of MalathionResidue in Tomato
In this a study tomato sampleswere collected from Khartoum, extracted with acetone, dichloromethane and petroleum ether (1:1:1) and cleaned up by florisil column. Malathion quantitative determination is carried out by ga...
Convenient one-step Synthesis of Substituted Pyrano[2,3-c]isoquinoline (2-amino-4H-chromenes) via three Component Reaction between Alkyl isocyanides and Dialkyl Acetylenedicarboxylate in the presence of 3-Hydroxyisoquinoline
The reactive intermediate generated by the addition of alkyl isocyanides to dialkyl acetylenedicarboxylate are trapped by 3-hydroxyisoquinoline to produce highly functionalized 4H-chromenes in 83-92% yields.
Experimental studies on the performance of a large-scale Solar Greenhouse dryer for banana in India A case study
Solar greenhouse drying is useful in conserving energy utilizing natural source of energy from sun and to speed up the process of drying crops. The main problem with unsold crops with farmers is addressed by solar...
Optimisation of solar panel efficiency for Solar powered vehicle
The aim of the project is to increase the efficiency of the solar panel. Solar technology offers great potential in terms of supplying the world’s energy needs. The effective way of utilizing sunlight with solar energy...