ACTIVITY OF GULURONATE LYASE IN THE ENHANCED PRODUCTION OF BACITRACIN A IN THE LIQUID CULTURES OF BACILLUS LICHENIFORMIS NCIMB 8874
Journal Title: World Journal of Pharmaceutical and life sciences - Year 2017, Vol 3, Issue 10
Abstract
This research reports for the first time the presence and the activity of guluronatelyase in the elicited (using oligoguluronate) liquid cultures of Bacillus licheniformis in the enhanced production of bacitracin A. Oligoguluronate (OG) prepared by partial acid hydrolysis of sodium alginate was added aseptically in concentrations ranging from 50 to 200 mg/L to cultures of Bacillus licheniformis (control cultures and cultures at 0hour (50 mg/L) and 24hour (200 mg/L)). Enhanced production of bacitracin A was observed at 32hour fermentation period with the highest production of bacitracin A obtained in the 0hour cultures (when 50 mg/L of OG was added at 0 hour) followed by the 24hour cultures to which 200 mg/L of OG was added. Results also showed, for the first time, that the enzyme guluronate lyase is produced in the cultures of Bacillus licheniformis when OG is added to the cultures and that the increase in the activity of this enzyme was concomitant with the bacterial growth, protein concentration of the cultures and the enhanced production of bacitracin A. The discovery of the presence of this enzyme (guluronatelyase) in the enhanced bacitracin A production from cultures of B. licheniformis could lead to potential applications of the methodology for large-scale production of bacitracin A in the future.
Authors and Affiliations
Constancy Prisca Aleru
Keywords
Related Articles
- EP ID EP649673
- DOI -
- Views 136
- Downloads 0
HYPERSPLENISM AND LIVER CIRRHOSIS
Objective: To determine the frequency of cases with hypersplenism presenting with liver cirrhosis. Methodology: This was a cross sectional study conducted at Sheikh Zayed Hospital, Rahim Yar Khan. In this study the diagn...
BENEFICIAL EFFECT OF ESTABLISHING THERAPEUTIC DRUG MONITERING IN IRAQI HOSPITALS
Background: The present research paper has been focused on the therapeutic drug monitoring (TDM) which refers to the individualization of dosage by maintaining plasma or blood drug concentrations within a target range (t...
LEGUMES OF SOLAN DISTRICT OF HIMACHAL PRADESH
The present study entitled “Legumes of Solan district of Himachal Pradesh” is carried out in different localities of Solan district of Himachal Pradesh to document the floral wealth of members belonging to family Legumin...
PERICARDIAL EFFUSION IN CASES OF MYOCARDIAL INFARCTION
Objective: To determine the frequency of pericardial effusion in cases of Myocardial Infarction. Methodology: This was a cross sectional study that was carried out at Lahore General Hospital, Lahore during November 2017...
TRANSDERMAL DRUG DELIVERY SYSTEM
The method by which a drug is delivered can have a significant effect on its efficacy. Some drugs have an optimum concentration range within which maximum benefit is derived, and concentrations above or below this range...