Activity of Sauropus androgynus L. leaf extract against inflammation and its immunomodulatory effect in Swiss albino mice.
Journal Title: International Journal of Advanced Biotechnology and Research. - Year 2016, Vol 7, Issue 2
Abstract
ABSTRACT In the present study, we have evaluated the anti-inflammatory and immunomodulatory potential of the methanol extract of Sauropus androgynus leaf. The Sauropus androgynus leaf extract (SALE) were ingested orally (p.o) in the form of suspension in 0.5% tween 80 in two different doses, 250 and 500 mg/kg body weight. The antiinflammatory activity of SALE treatment was checked in acute and chronic inflammation in mice model induced by carrageenan and formaldehyde respectively. Immunomodulatory activity was checked both at cellular and humoral levels. Cellular immunity was evaluated by Delayed type Hypersensitivity (DTH), neutrophil adhesion test and carbon clearance assay, whereas, humoral immunity was analyzed by heamagglutination antibody (HA) titer assay. Following total WBC count, body weight and relative immune organ weight were analyzed. Acute oral toxicity study concluded that SALE was safe at 2500 mg/kg. The results showed that administration of SALE (500 mg/kg bw) significantly reduced oedema formation, the reduction was similar to diclofenac. The same dose of SALE caused a significant increase in HA titer in response to sheep red blood cells (SRBC) when compared to control group, and a decrease in DTH (footpad thickness) in response to challenge with SRBC sensitized hosts and also caused increases in WBC counts. Administration of SALE enhanced the relative organ weight and showed a significant (p≤0.01) increase in percentage neutrophil adhesion to nylon fibers and phagocytic activity. Based on the results, we conclude that SALE has a potent immunomodulatory potential but also could significantly impact upon inflammation reactions.
Authors and Affiliations
Ramesh Kumar. P and Philomena George
Keywords
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