Acute 1L-1β Luciferase Signal and Immune Response to Surface Modified Polyurethane-Type Shape Memory Polymers: A Pilot Study
Journal Title: Biomedical Journal of Scientific & Technical Research (BJSTR) - Year 2019, Vol 14, Issue 4
Abstract
Polyurethane-type Shape Memory Polymers (SMPU) are promising biomaterials due to the ability to change shape by external stimulus. However, SMPU suffer from hydrophobicity and bio-inertness which restricts their possibilities as implantable biomedical applications. Plasma Immersion Ion Implantation (PIII) is a one-step covalent method to functionalise SMPU and to create a tuneable, biocompatible surface by attaching biomolecules. In this pilot study, collagen was used to immobilise on PIII treated SMPU surfaces and SMPU ± PIII and ± collagen were subcutaneously implanted in 1L-1β mice. Real-time 1L-1β luciferase signal was detected at day 1 and acute inflammatory responses at day 7 were determined using H&E staining histologically. The results indicate significantly less host immune responses to PIII treated SMPU with collagen coating. This pilot study provides a solid foundation for further acute/chronic study in vivo. As one of smart materials, Shape Memory Polymers (SMP) have been investigated extensively for biomedical applications because of their shape memory properties [1], such as vascular stents, aneurysm occlusion devices, and clot extraction devices [2-5]. Polyurethane-type Shape Memory Polymers (SMPU), one of the most common SMP, have the advantage of a glass transition temperature closed to body temperature [6], facilitating the activation of shape memory recovery within the body. However, SMPU still suffer from inherent hydrophobicity and bio-inertness [7]. This may bring about immune reactions and poor tissue integration. Therefore, creating bioactive interfaces prior to implantation is of great importance [8]. Plasma surface treatments, such as Plasma Immersion Ion Implantation (PIII), are efficient methods to change surface properties without changing bulk properties, involving wettability, chemical structure, and hardness. These changes can contribute to the improvement of interactions between biomolecules and biomaterial surfaces [9]. Additionally, PIII enables one-step covalent attachment of biomolecules without the participance of any chemical reagents [10], facilitating the applications of SMPU in biomedical industry easily. It has been documented that protein can be covalently immobilised on PIII treated polymer surfaces, leading to the improvement of cell adhesion, proliferation, and viability on the interfaces [11-13]. In our previous study, covalent immobilisation of collagen has been observed on PIII treated SMPU surfaces in vitro [14]. Therefore, we aim to conduct a pilot study to study biocompatibility of SMPU ± PIII and ± collagen in vivo. The acute 1L-1β luciferase signal was detected in real time and acute inflammatory response to the implanted SMPU was determined at the histopathological level. Polyurethane-type Shape Memory Polymers (SMPU) were purchased from DiAPLEX Co., Ltd (Tokyo, Japan). SMPU incorporates hard segments of 4, 4’ Diphenylmethane Diisocyanate (MDI) and polyether type soft segments of polypropylene glycol and polyethylene glycol, as described [15].
Authors and Affiliations
Xinying Cheng, Jian Fei, Lin Ye, Marcela MM Bilek, Shisan Bao
Keywords
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- EP ID EP594370
- DOI 10.26717/BJSTR.2019.14.002579
- Views 180
- Downloads 0
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