Acute and chronic effects of gold nanoparticles on sperm parameters and chromatin structure in Mice
Journal Title: International Journal of Reproductive BioMedicine - Year 2016, Vol 14, Issue 10
Abstract
Background: The particles in the range of 1-100 nm are called nanoparticles. Gold nanoparticle is one of the most important metal nanoparticles with wide usage. Objective: This study investigated the effects of gold nanoparticles on sperm parameters and chromatin structure in mice. Materials and Methods: In this experimental study, 72 male bulb-c mice were divided into 9 groups including: 4 Sham groups (Sc 1-4), 4 experimental groups (Au 1-4), and 1 control group (C). Experimental groups received 40 and 200 µg/kg/day soluble gold (Au) nano-particles for 7 and 35 days, by intra peritoneal injection, respectively. Sham groups were treated with 1.2 mM sodium citrate solution with 40 and 200 µg/kg/day doses for same days and control group did not receive any materials. Motility and Morphology of spermatozoa were analyzed. Chromatin quality was also evaluated using AB (Aniline blue), TB (Toluidine blue) and CMA3 (Chromomycin A3) staining methods. Results: The sperm analysis results showed that motility and morphology of sperm in experimental groups (especially in groups that have been treated for 35 days with nano-particles) had significant decrease in comparison with control group. TB, AB and CMA3 results showed a significant increase in abnormal spermatozoa from all Au-treated groups. Conclusion: Gold nano-particles firstly can reduce the sperm parameters such as motility and normal morphology and secondly affect sperm chromatin remodeling and cause the increase instability of chromatin and also increase the rate of sperm DNA damage. These deleterious effects were more obvious in maximum dose and chronic phase.
Authors and Affiliations
Mahsa Nazari, Ali Reza Talebi, Mohammad Hosseini Sharifabad, Abolghasem Abbasi, Arezoo Khoradmehr, Amir Hossein Danafar
Keywords
Related Articles
- EP ID EP190799
- DOI -
- Views 111
- Downloads 0
Impacts of morphine addiction on spermatogenesis in rats
Background: There are numerous investigations on wide range of issues that disrupt regulatory spermatogenesis, individuals who are exposed to drug abuse faced infertility and immature spermatogenesis. Objective: The aim...
Comparison of clinical performance of antigen based-enzyme immunoassay (EIA) and major outer membrane protein (MOMP)-PCR for detection of genital Chlamydia trachomatis infection
Background: Chlamydia trachomatis is the most common sexually transmitted bacterial pathogen worldwide. Early detection and treatment of C.trachomatis genital infection prevent serious reproductive complications. Object...
The protective effect of vitamin E on rats’ ovarian follicles following an administration of diazinon: An experimental study Zinat Sargazi1 M.Sc., Mohammad Reza Nikravesh2 Ph.D.,
Background: Diazinon (DZN) is an organophosphate insecticide that has been widely utilized in agriculture all over the world and caused many negative effects on different species such as plants and animal species, especi...
Association between HLA-E gene polymorphism and unexplained recurrent spontaneous abortion (RSA) in Iranian women
Background: Human leukocyte antigen-E (HLA-E)is a non-classical major histocompatibility complex (MHC) class I antigens which expressed on extra villous cytotrophoblast, which interacts with NKG2A, is an inhibitory recep...
Association of leptin and insulin resistance in PCOS: A case-controlled study
Background: Endocrine abnormalities related to polycystic ovary Syndrome (PCOS) are important problems. Objective: To compare serum leptin levels between infertile women with and without PCOS. To rank sensitivity of six...