Acute Toxicity of Methanol Extract of Cola nitida Treatment on Antioxidant Capacity, Hepatic and Renal Functions in Wistar Rats
Journal Title: International Journal of Biochemistry Research & Review - Year 2016, Vol 13, Issue 4
Abstract
Aim: The study investigated the effect of methanol crude extract of Cola nitida on some liver function enzymes and antioxidant in wistar rats. Place and Duration of Study: The study was carried out at the Department of Biochemistry, College of Natural Sciences, Michael Okpara University of Agriculture, Umudike, Nigeria, in the year 2015. Methodology: LD50 was carried out by administering 1000, 2000, 4000, 5000, 8000 and 10000 mg/kg of the Cola nitida extract to twenty-four animals of four animals per group. For biochemical analysis, fifteen animals were divided into three groups of five animals each, group A served as control group, group B and C received 300 and 500 mg/kg B.W of Cola nitida respectively. Results: Result indicated an LD50 of 6320 mg/kg B.W. Catalase, revealed a significant (P =.05) decrease in test group (36.7±21.1) for 300 mg/kg and (29.46±18.8) for 500 mg/kg body weight when compared to the control group (59.7±4.3). Superoxide dismutase concentration was also significantly (P =.05) lower in the 500 mg/kg group (10.32 ± 4.90) when compared to the control group (24.94±2.39). GSH concentration was significantly (P =.05) lower in the 500 mg/kg group (21.1±1.91) when compared to the control group (23.3±1.28). Result for MDA significantly (P =.05) decreased in the control group (0.08±0.03) when compared to the 300 mg/kg group (0.11±0.00) and 500 mg/kg group (0.12±0.03). ALT and AST concentrations were significantly (P<0.05) higher in the 500 mg/kg group (43.60+20.47 and 30.60+20.99) when compared to the control group (23.40+3.36 and 11.20+5.84). Conclusion: Result obtained from this study showed that administration of Cola nitida induced lipid peroxidation, and also some signs of hepatotoxicity.
Authors and Affiliations
Ejiofor U. Emmanuel, Shirley O. Ebhohon, Obike C. Adanma, Oriaku C. Edith, Ogbonnaya N. Florence, Ineama Chioma, Uroko I. Roberts, Omeh Y. Ndukaku
Keywords
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- EP ID EP351161
- DOI 10.9734/IJBCRR/2016/28593
- Views 108
- Downloads 0
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