Acute treatment of constant darkness increases the efficiency of ATP synthase in rat liver mitochondria
Journal Title: Annals of Hepatology - Year 2009, Vol 8, Issue 4
Abstract
The circadian oscillations of many physiological processes provide an endogenous temporal program for the adaptive synchronization of mammals to the fluctuating external world. The lack of exposure to light causes the circadian system to undergo a process of dark adaptation similar to dark adaptation in the visual system. The aim of the present work was investigate the effect of acute treatment of constant darkness on mitochondrial ATP synthase activities and membrane fluidity in liver from male rat. We found that ATP synthase activity was not changed by the treatment. However ATPase activity and membrane fluidity were significantly diminished and pH gradient driven by ATP hydrolysis was incremented, in comparison from samples from rats kept on normal light/dark cycles. Additionally, the treatment of constant darkness diminishes the passive proton permeability of the inner mitochondrial membrane. In conclusion constant darkness induces a more efficient coupling between proton transport and catalysis, and increment the efficiency of the enzyme because the ratio of ATP synthase/ATPase activity was higher. These results exhibited the physiological adaptation of liver mitochondria to acute treatment of constant darkness in order to satisfy the cellular energy demand.
Authors and Affiliations
Ana Ramírez-Iñiguez, Genaro Ortiz, Mohammed El Hafidi, Ernesto Rodríguez-Macías, Pacheco-Moisés Paul
Keywords
Related Articles
- EP ID EP77894
- DOI -
- Views 151
- Downloads 0
The Changing Face of the Diagnosis of Chronic and Malignant Liver Diseases: Potential New Biomarkers
The early diagnosis of primary sclerosing cholangitis, hepatocellular carcinoma, and cholangiocarcinoma is often challenging. In a recent study in 134 patients (Arbelaiz, Hepatology 2017; 66:1125-1143), it was reported t...
What constitutes liver failure after transjugular intrahepatic portosystemic shunt creation? A proposed definition and grading system
Background and rationale for the study. There is currently no definition of post-transjugular intrahepatic portosystemic shunt(TIPS) liver failure (PTLF), which constitutes a barrier to standardization of TIPS results r...
Influence of Famine in Women with Non-Alcoholic Fatty Liver Disease
-
Hepatitis C virus infection in patients with primary biliary cirrhosis
[b]Background and aim[/b]. The aim of this study is to evaluate the role of hepatitis C virus (HCV) infection in patients with primary biliary cirrhosis (PBC). [b]Material and methods[/b]. On the basis of a retrospective...
Swallowed chicken bone migration into the liver