Adaptation of primer extension method for monitoring of scrapie-associated genotypes in sheep PRNP gene
Journal Title: Animal Science Papers and Reports - Year 2012, Vol 30, Issue 2
Abstract
The paper describes adaptation of primer extension method for genotyping scrapie-associated codons 136, 141 and 156 in sheep PRNP gene. As the method failed to genotype the polymorphism in codon 171 in some samples, this polymorphism was excluded from the assay. Such genotyping problems were not observed in earlier studies describing the method and cannot be easily explained. However, full accordance of genotypes in codons 136, 141 and 156 with sequencing results was obtained and thus the method can be recommended for routine application. Simultaneously, we strongly recommend to pay special attention when using the minisequencing method for genotyping codon 171 in sheep PRNP.
Authors and Affiliations
Grzegorz Smołucha, Artur Gurgul, Agata Piestrzyńska-Kajtoch, Barbara Rejduch
Keywords
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