Adipose Derived Stem Cells Conditioned Media in Combination with Bioceramic-Collagen Scaffolds Improved Calvarial Bone Healing in Hypothyroid Rats
Journal Title: UNKNOWN - Year 2017, Vol 19, Issue 5
Abstract
Background: Tissue engineering is an available treatment for large bone defects. The therapeutic effects of mesenchymal stem cell (MSC) are mostly attributed to secretion of many cytokines and growth factors. Many factors of MSC secretions accumulate in a conditioned medium and these factors recruit native cells into a defect site to generate new bone tissues. Objectives: The aim of this study was to evaluate the influence of adipose tissue derived MSCs-conditioned medium (ADMSC-CM) on bone repair of rats with critical - size calvarial defect. Methods: This experimental study was performed at Shahid Beheshti University of Medical Sciences, Tehran, Iran, from 2016 to 2017. Conditioned medium was collected from healthy rat adipose tissue derived MSC (ADMSC) at passage four. Calvarial bone defect was created in hypothyroid rats using a dental bur. Sampling was taken by the linear-mono-gram method to determine sample size (n = 6 per group). The rats were divided randomly into four groups based on graft material as follows: empty defect, scaffold (Bio-Oss / type I collagen gel), scaffold / ADMSCs, scaffold /ADMSC- CM. Evaluations weremadeat 4 and 8 weeks after surgery using stereological analysis. Results: Histological analysis at 8 weeks indicated that the newly regenerated tissue almost covered the defect in the ADMSC-CM group. Stereological analysis showed that ADMSC-CM increased regenerated bone and numbers of osteocytes and osteoblasts compared with the defect and scaffold groups (P < 0.05). Also, bone regeneration was more effective in animals treated with ADMSC-CM than in those received ADMSC. Conclusions: These results suggest an important role for ADMSC-CM in bone regeneration, through trophic impact of its cytokines andgrowthfactors that induce native cell proliferationandmigration into the defect. Thus,ADMSC-CMseemstohavegoodpotential for application in bone tissue regeneration, in the cases of hypothyroidism.
Eosinophilic Granulomatosis with Polyangiitis and Spinal Subdural Hematoma: A Case Report and Review of the Literature
Introduction: Eosinophilic granulomatosis with polyangiitis (EGPA), formerly known as Churg-Strauss syndrome, is a rare systemic vasculitis. Central nervous system (CNS) involvement is described in rare cases. Acute spin...
Designing and Psychometric Evaluation of Questionnaire of Influencing Factors of Nursing Students’ Clinical Judgment: A Mixed Method Study in an Iranian Context
Background: Identification of the influencing factors of nursing students’ clinical judgment (CJ) facilitates the achievement of this important educational outcome. However, no tool is so far designed to assess it in the...
Depression Improvement Among Patients with HIV and Endocrine Dysfunction After hormone Therapy
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The Role of Quercetin in Gene Expression of GluR1 Subunit of AMPA Receptors, and NR2A and NR2B Subunits of NMDA Receptors in Kainic Acid Model of Seizure in Mice
Background: Flavonoids are recently being recognized for their important biological effects. Quercetin is a flavonoid that has several pharmacological properties including anti-inflammatory, neuroprotective, and anticonv...
The Prevalence of Dysmenorrhea in Iran: A Systematic Review and Meta-Analysis
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