Advantage of the use of oxyreductive dye and automated reading in the determination of pharmaceutical activity in bacteria of medical importance
Journal Title: Revista Uningá - Year 2023, Vol 60, Issue 1
Abstract
Urinary Tract Infections (UTIs) are frequent bacterial infections in Brazil, with the Escherichia coli and Staphylococcus aureus bacilli being the most common. In the context of the pandemic caused by the SARS-CoV-2 virus, hospital infections have gained prominence, as they have been considered aggravating factors for the disease and have increased mortality rates. In this context, rapid and accurate diagnosis is essential in order to reduce costs, therapeutic errors, and development of resistant strains due to the inappropriate use of antimicrobials. The antibiotic sensitivity profile is determined by the Minimum Inhibitory Concentration (hereinafther, MIC), and the result is obtained from the observation of turbidity in the microplates with the naked eye. This technique has been improved with the use of redox indicators that can facilitate the interpretation of results through color changes. Thus, this study aimed to evaluate the performance of three dyes incorporated in the MIC for E. coli and S. aureus bacteria, in order to adopt them in routine laboratory work and to provide a better cost-benefit ratio as well as more appropriate reading. The visualization was based on the color change of the dyes and in the analysis of the absorbances emitted by a spectrophotometer, which were used in specific calculations for graph preparation. The results showed that it was possible to determine the MIC of the above-described bacteria in a minimum time of 40 minutes and to conclude that the indicators and automated reading facilitate the interpretation of bacterial growth.
Authors and Affiliations
Ana Beatriz Miranda, Luiz Henrique Reinert, Jean Eduardo Meneguello, Andressa Lorena Ieque, Vera Lucia Dias Siqueira, Regiane Bertin de Lima Scodro, Rosilene Fressatti Cardoso, Katiany Rizzieri Caleffi-Ferracioli.
Keywords
Related Articles
- EP ID EP718918
- DOI 10.46311/2318-0579.60.eUJ4398
- Views 37
- Downloads 0
Comorbidities and deaths by COVID-19 in Brazil
The new coronavirus outbreak in November 2019 quickly became an international public health emergency. Some studies demonstrate that the poor prognosis of the disease is directly related to the presence of comorbidities,...
Walking paths: Therapeutic itineraries of women using alcohol and/or other drugs during their pregnancy-postpartum cycle
Pregnancy, childbirth and postpartum care is provided in accordance with gestational risk factors, such as use of alcohol and/or other drugs by pregnant women. The objective of this study was to trace the therapeutic iti...
Deciduous teeth stem cells, promising source of stem cells: narrative literature review
Among the most progressive types of medical-scientific research, the study with stem cells stands out. Stem cell therapy has emerged as an innovative model in the treatment of diseases and injuries, presenting numerous a...
Fractures in motorcycle accidents
Injuries and trauma caused by motorcycle accidents continue to have an important economic and social impact. Therefore, this study aimed to estimate the prevalence of fractures stemmed from traffic accidents, involving m...
The importance of dental prenatal care in infant health: A literature review
The physiological changes resulting from pregnancy and habits during pregnancy motivate interest about the need for dental prenatal care, in order to understand the repercussions on the oral health of the mother and the...