“ISOLATION AND CHARACTERIZATION OF LACTOSE AND NON- LACTOSE FERMENTING BACTERIA FROM TERTIARY CARE HOSPITAL AND THEIR ANTIMICROBIAL SUSCEPTIBILITY TESTâ€
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2017, Vol 10, Issue 2
Abstract
Objective: The purpose of the present study was to identify the fermenting and non-fermenting gram negative bacteria from the tertiary care hospital.Methods: The conventional method of identification by biochemical analysis and antibiotic susceptibility test was performed by Kirby-Bauer disc diffusion method. Furthermore, analysis of microbes was done by Vitek-2.Results: 424strains of lactose fermenting and non-lactose fermenting gram negative bacilli were isolated from 3097 clinical samples. From the total lactose fermenting bacteria Escherichia coli was the predominant isolate accounting for 50.94% specimens, followed by Klebsiella pneumonia 27.59% and Enterobacter 0.47%. From the total non-lactose fermenting gram negative bacilli Acinetobacter baumannii was the predominant isolate accounting for 12.73% specimens followed by Pseudomonas aeroginosa 6.13%, other isolates were Stenotrophomonas maltophilia 1.17% , Burkholderia cepacia 0.94%. In the present study male were more infected than female. The study also showed that lactose fermenting bacteria were more infectious than non lactose-fermenting bacteria and isolates were from urine samples.Conclusion: Both Non-Lactose Fermenting Gram Negative Bacilli and Lactose Fermenting Gram Negative Bacilli were found to be major contaminants, and are important pathogenic bacteria causing wide range of infections in the tertiary care hospital.Keywords: Lactose fermenting gram negative bacteria, Vitek-2, Tertiary Care Hospital, Kirby-Bauer Disc Diffusion, Lactose non-fermenting gram negative bacteria Â
Authors and Affiliations
Kumud Bala, Ridhima Wadhwa, Rachana Bohra
AN IN-VITRO ANTIBIOFILM ACTIVITY OF CHLORELLA VULGARIS
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COMPARATIVE STUDY OF METABOLITES AND ANTIMICROBIAL ACTIVITIES OF ESSENTIAL OILS EXTRACTED FROM THREE AMOMUM SUBULATUM CULTIVARS
Objective: In Sikkim (India), Seremna is a highly growing cultivar of Amomum subulatum at lower altitudes. Other popular cultivated varieties are Varlangy and Sawney in the same state but at different altitude. In this s...
ANTIMICROBIAL AND CHITINASE INHIBITORY ACTIVITIES OF 7-BENZAMIDOCOUMARINS
Objective: The aim of this study was to examine in vitro antimicrobial activity of a series of 7-benzamidocoumarin derivatives against three Gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus, and Bacillus...
PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ACTIVITY OF PORTULACA QUADRIFIDA LINN.
Objective: The objective of the study was to investigate in vitro antimicrobial activity against enterotoxigenic Escherichia coli and Bacillus subtilis and preliminary phytochemical screening of the leaves of Portulaca q...
OPTIMIZATION OF BIOMASS CULTURE YIELD AND L-DOPA COMPOUND IN THE CALLUS CULTURE FROM COTYLEDONARY LEAVES OF MUCUNA PRURIENS
ABSTRACTObjective: Â The objective of the present study was to evaluate the optimization of callus biomass culture yield and high-performance liquid chromatography (HPLC) analysis of L-DOPA compound in the callus culture...