Age influence on mice lung tissue response to [i]Aspergillus fumigatus[/i] chronic exposure
Journal Title: Annals of Agricultural and Environmental Medicine - Year 2015, Vol 22, Issue 1
Abstract
[b]Introduction and objective[/b]. Exposure to conidia of [i]Aspergillus fumigatus[/i] was described as a causative factor of a number of the respiratory system diseases, including asthma, chronic eosinophilic pneumonia, hypersensitivity pneumonitis and bronchopulmonary aspergillosis. The study investigates the effects of the repeated exposure to [i]A. fumigatus[/i] in mice pulmonary compartment. Our work tackles two, so far insufficiently addressed, important aspects of interaction between affected organism and[i] A. fumigatus[/i]: 1) recurrent character of exposure (characteristic for pathomechanism of the abovementioned disease states) and 2) impact of aging, potentially important for the differentiation response to an antigen. [b]Materials and methods[/b]. In order to dissect alterations of the immune system involved with both aging and chronic exposure to [i]A. fumigatus[/i], we used 3- and 18-month-old C57BL/6J mice exposed to repeated[i] A. fumigatus[/i] inhalations for 7 and 28 days. Changes in lung tissue were monitored by histological and biochemical evaluation. Concentration of pro- and anti-inflammatory cytokines in lung homogenates was assessed by ELISA tests. [b]Results and conclusions. [/b]Our study demonstrated that chronic inflammation in pulmonary compartment, characterized by the significant increase of proinflammatory cytokines (IL1, IL6, IL10) levels, was the dominant feature of mice response to repeated [i]A. fumigatus[/i] inhalations. The pattern of cytokines’ profile in the course of exposure was similar in both age groups, however in old mice the growth of the cytokines’ levels was more pronounced (especially in case of IL1).
Authors and Affiliations
Marta Kinga Lemieszek, Jacek Dutkiewicz, Marcin Golec, Marco Chilosi, Czesława Skórska, Francois Huaux, Chiara Pastena, Federica Pedica, Jolanta Sitkowska, Wiesława Lisowska, Grażyna Cholewa, Barbara Mackiewicz, Anna Góra-Florek, Rolf Ziesche, Janusz Milanowski
Keywords
Related Articles
- EP ID EP80917
- DOI -
- Views 132
- Downloads 0
Analysis of the phenomenon of attempted suicides in 1978-2010 in Poland, with particular emphasis on rural areas of Lublin Province
The increasing quality of life of modern man should go hand-in-hand with reducing the scale of the problem of attempted suicides. During the last 55 years, the World Health Organization has recorded an increase in the nu...
Effect of selected socio-demographic, clinical and biochemical factors on self-reported quality of life among post-menopausal women with osteoporosis
Introduction: The goal of health care with relation to women with osteoporosis is the prevention of fractures, maintenance of independence, and good quality of life. Objective: To discover how selected socio-demographic,...
Cardiac channelopathies – A too frequently trivialized problem?
Channelopathies are a genetically conditioned group of disorders related with defects in ion channels, responsible for the occurrence of dangerous abnormal heart rhythm in a structurally normal heart. These diseases are...
Engagement of children in agricultural work activities--scale and consequences of the phenomenon.
The purpose of the study was investigate the scale and consequences of the engagement of children in agricultural work activities in Poland. The study covered 1,006 children aged 12-14, who completed an audience question...
The influence of factors of work environment and burnout syndrome on self-efficacy of medical staff
[b]Introduction and objective.[/b] Conditions of a healthy, friendly and safe work environment and proper work organisation increase self-efficacy and decrease or eliminate the factors causing the occurrence of burnout s...