Age influence on mice lung tissue response to [i]Aspergillus fumigatus[/i] chronic exposure
Journal Title: Annals of Agricultural and Environmental Medicine - Year 2015, Vol 22, Issue 1
Abstract
[b]Introduction and objective[/b]. Exposure to conidia of [i]Aspergillus fumigatus[/i] was described as a causative factor of a number of the respiratory system diseases, including asthma, chronic eosinophilic pneumonia, hypersensitivity pneumonitis and bronchopulmonary aspergillosis. The study investigates the effects of the repeated exposure to [i]A. fumigatus[/i] in mice pulmonary compartment. Our work tackles two, so far insufficiently addressed, important aspects of interaction between affected organism and[i] A. fumigatus[/i]: 1) recurrent character of exposure (characteristic for pathomechanism of the abovementioned disease states) and 2) impact of aging, potentially important for the differentiation response to an antigen. [b]Materials and methods[/b]. In order to dissect alterations of the immune system involved with both aging and chronic exposure to [i]A. fumigatus[/i], we used 3- and 18-month-old C57BL/6J mice exposed to repeated[i] A. fumigatus[/i] inhalations for 7 and 28 days. Changes in lung tissue were monitored by histological and biochemical evaluation. Concentration of pro- and anti-inflammatory cytokines in lung homogenates was assessed by ELISA tests. [b]Results and conclusions. [/b]Our study demonstrated that chronic inflammation in pulmonary compartment, characterized by the significant increase of proinflammatory cytokines (IL1, IL6, IL10) levels, was the dominant feature of mice response to repeated [i]A. fumigatus[/i] inhalations. The pattern of cytokines’ profile in the course of exposure was similar in both age groups, however in old mice the growth of the cytokines’ levels was more pronounced (especially in case of IL1).
Authors and Affiliations
Marta Kinga Lemieszek, Jacek Dutkiewicz, Marcin Golec, Marco Chilosi, Czesława Skórska, Francois Huaux, Chiara Pastena, Federica Pedica, Jolanta Sitkowska, Wiesława Lisowska, Grażyna Cholewa, Barbara Mackiewicz, Anna Góra-Florek, Rolf Ziesche, Janusz Milanowski
Keywords
Related Articles
- EP ID EP80917
- DOI -
- Views 112
- Downloads 0
Aerobiological study in east-central Iberian Peninsula: pollen diversity and dynamics for major taxa.
A study was made of airborne pollen counts in Cuenca (east-central Iberian Peninsula, Spain), using data obtained over a 3-year period (2008-2010). This is the first such study carried out in the World Heritage city of C...
Harmonisation of standards related to limiting chemical risk associated with work processes
The presented paper tackles the issue of risk factors specific to work processes that involve the presence of chemicals. The reason that supports the present approach is the fact that the risks most likely to affect heal...
Occurrence and antimicrobial resistance of Salmonella spp. isolated from food other than meat in Poland
Introduction and objectives. Antimicrobial resistance of pathogenic bacteria can result in therapy failure, increased hospitalization, and increased risk of death. In Poland, [i]Salmonella[/i] spp. is a major bacterial a...
Analyses of hospitalization of diabetes mellitus patients in Poland by gender, age and place of residence
Aim. The purpose of this study was to analyze the hospitalization of diabetes mellitus patients in Polish hospitals in 2005-2009.Materials and Methods. Data was taken from a nationwide database, kept at the National Inst...
Raised concentrations of lipid peroxidation products (LPO) in pregnant women with impaired glucose tolerance
introduction. Lipid peroxidation (LPO) results from oxidative damage to membrane lipids. Whereas LPO rises in normal pregnancy, the effect of gestational diabetes mellitus (GDM) on this process has not been clearly defin...