AISLAMIENTO DE Rickettsia bellii A PARTIR DE GARRAPATAS Amblyomma ovale Y Amblyomma incisum PROCEDENTES DEL SUR DE BRASIL
Journal Title: Revista MVZ Cordoba - Year 2008, Vol 13, Issue 2
Abstract
Objective. To isolate and characterize rickettsiae from the ticks Amblyomma ovale and Amblyomma incisum collected in the state of São Paulo. Materials and methods. Adult, free-living A. ovale and A. incisum were collected in an Atlantic rainforest area in the state of São Paulo, Brazil. Each tick was tested using the hemolymph assay; samples from positive ticks were placed in shell vials in order to isolate rickettsiae and subsequently grown in Vero cells. Amplification of three rickettsial genes (gltA, htrA and ompA) was attempted using polymerase chain reaction (PCR) for each isolate obtained. Amplicons were subsequently sequenced. Results. A total of 388 A. incisum and 50 A. ovale were collected. Only one A. incisum and one A. ovale were hemolymph-test positive. Rickettsiae were successfully isolated from these ticks; however establishment in Vero cell culture was successful only for the isolate from A. ovale. Bacterial contamination in the first cell passage of the A. incisum isolate precluded successful isolation of the organism. PCR products were obtained with the gltA and htrA primers for the two isolates, but no product was obtained with the ompA primers. By BLAST analysis, partial gltA and htrA sequences of isolates from A. ovale and A. incisum were similar to the corresponding sequences of R. bellii. Conclusions. This is the first report of R. bellii infecting A. incisum and the first successful isolation from A. ovale.
Authors and Affiliations
Richard Pacheco, Ph. D *| University of São Paulo, Faculty of Veterinary Medicine, São Paulo, SP, Brazil.Correspondencia: pachecorc@hotmail.com, Simone Rosa, MVZ| University of São Paulo, Faculty of Veterinary Medicine, São Paulo, SP, Brazil., Leonardo Richtzenhain, Ph. D| University of São Paulo, Faculty of Veterinary Medicine, São Paulo, SP, Brazil., Matias P. J. Szabó, Ph. D| Federal University of Uberlândia, Faculty of Veterinary Medicine, Uberlândia, MG, Brazil, Marcelo B. Labruna, Ph. D| University of São Paulo, Faculty of Veterinary Medicine, São Paulo, SP, Brazil.
Keywords
Related Articles
- EP ID EP3094
- DOI -
- Views 412
- Downloads 22
Quality Index Method developed for gutted and ungutted red tilapia (Oreochromis ssp)
Objective. To develop a Quality Index Method (QIM) for gutted and ungutted red tilapia from aquaculture ponds. Materials and methods. 40 specimens of gutted red tilapia and 40 ungutted ones were located in foam polyethyl...
Bovine otitis by Rhabditis bovis in Cordoba, colombia. Report of two cases
Two cases of pure Gyr bred bovines, treated at the outpatient medical-surgical clinic for large animals of the Faculty of Veterinary Medicine and Animal Husbandry, at the University of Cordoba, Colombia are reported. Dur...
Bats associated to a cattle farm in Córdoba (Colombia)
Objective. Knowing the bat species associated with tropical dry forest remaining in a system of extensive livestock in a farm in the department of Cordoba. Materials and methods. Sampling of 35 nights during the dry seas...
Entrance of parrots to the center of attention of wildlife (CAV-CVS), during the years 2007-2009
Objetive. The entrance dynamics of family Psittacidae to the Wildlife Attention and Valuation Center (CAV - CVS) was determined. Materials and methods. Data were obtained between 2007 to 2009. A frequency analysis for th...
Effect on weight gain, rumen bioactivity and rumen pH in weaned sheep using fresh rumen fluid from cattle
Objetive. To determine the effect on weight gain, rumen bioactivity and rumen pH in weaned sheep using fresh rumen fluid (FRF) from cattle. Materials and methods. Nine weaned sheep with (aproximated same age and weight),...