AISLAMIENTO DE Rickettsia bellii A PARTIR DE GARRAPATAS Amblyomma ovale Y Amblyomma incisum PROCEDENTES DEL SUR DE BRASIL
Journal Title: Revista MVZ Cordoba - Year 2008, Vol 13, Issue 2
Abstract
Objective. To isolate and characterize rickettsiae from the ticks Amblyomma ovale and Amblyomma incisum collected in the state of São Paulo. Materials and methods. Adult, free-living A. ovale and A. incisum were collected in an Atlantic rainforest area in the state of São Paulo, Brazil. Each tick was tested using the hemolymph assay; samples from positive ticks were placed in shell vials in order to isolate rickettsiae and subsequently grown in Vero cells. Amplification of three rickettsial genes (gltA, htrA and ompA) was attempted using polymerase chain reaction (PCR) for each isolate obtained. Amplicons were subsequently sequenced. Results. A total of 388 A. incisum and 50 A. ovale were collected. Only one A. incisum and one A. ovale were hemolymph-test positive. Rickettsiae were successfully isolated from these ticks; however establishment in Vero cell culture was successful only for the isolate from A. ovale. Bacterial contamination in the first cell passage of the A. incisum isolate precluded successful isolation of the organism. PCR products were obtained with the gltA and htrA primers for the two isolates, but no product was obtained with the ompA primers. By BLAST analysis, partial gltA and htrA sequences of isolates from A. ovale and A. incisum were similar to the corresponding sequences of R. bellii. Conclusions. This is the first report of R. bellii infecting A. incisum and the first successful isolation from A. ovale.
Authors and Affiliations
Richard Pacheco, Ph. D *| University of São Paulo, Faculty of Veterinary Medicine, São Paulo, SP, Brazil.Correspondencia: pachecorc@hotmail.com, Simone Rosa, MVZ| University of São Paulo, Faculty of Veterinary Medicine, São Paulo, SP, Brazil., Leonardo Richtzenhain, Ph. D| University of São Paulo, Faculty of Veterinary Medicine, São Paulo, SP, Brazil., Matias P. J. Szabó, Ph. D| Federal University of Uberlândia, Faculty of Veterinary Medicine, Uberlândia, MG, Brazil, Marcelo B. Labruna, Ph. D| University of São Paulo, Faculty of Veterinary Medicine, São Paulo, SP, Brazil.
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