ALPHA-AMYLASE INHIBITION AND MEMBRANE STABILIZING EFFECT OF THE STEM BARK OF MAESOBOTRYA DUSENII HUTCHINSON
Journal Title: International Journal of Pharmaceutical Sciences and Research (IJPSR) - Year 2019, Vol 10, Issue 11
Abstract
Introduction: Control of blood glucose level is a critical approach in the management of diabetes mellitus and its complications. This study investigated the α-amylase inhibition and erythrocyte membrane stabilization effect of extracts of Maesobotrya dusenii Hutch. Method: Powdered stem bark of M. dusenii (100g) was extracted with methanol. The plant material (400 g) was also successively macerated with N-hexane, ethyl acetate, and methanol. Phytochemical screening was conducted on the powdered stem bark. All the extracts were evaluated for alpha-amylase inhibition at 10, 20, 30, 40 and 50 µg/mL concentrations using acarbose as standard drug and erythrocyte membrane stabilization at 20, 40, 60 and 80 µg/mL concentrations using diclofenac as standard drug. Gas Chromatography-Mass Spectroscopy (GC-MS) of the hexane extract was analyzed. Result: Phytochemical screening showed the presence of carbohydrates, saponins, and triterpenoids. All the extracts showed a dose-dependent response on alpha-amylase inhibition in which crude methanol had a significant activity of 56.7% inhibition. N-Hexane, ethyl acetate, and crude methanol extracts showed erythrocyte membrane stabilizing effect, but crude methanol showed the most activity with 183.3% inhibition of erythrocyte membrane stabilization and IC50 of 70 µg/mL against diclofenac 28.4 µg/mL. The GC-MS revealed hexadecanoic acid and six other compounds that were fully characterized by database. Conclusion: The study showed that crude methanol extract has significant alpha-amylase inhibitory and anti-inflammatory activity than other extracts. The study justifies the use of M. dusenii in the management of diabetes.
Authors and Affiliations
S. Mikailu et al.
Keywords
Related Articles
- EP ID EP665756
- DOI 10.13040/IJPSR.0975-8232.10(11).5154-59
- Views 102
- Downloads 0
AUTHENTICATION OF SEVEN INDIAN TRADITIONAL TYPES OF THE HERBAL DRUG TERMINALIA CHEBULA WITH SCIENTIFIC UPDATES – AN ADVANCED INSIGHT
The fruit of Terminalia chebula is an essential drug used in single and in many compound formulations of Indian traditional systems like Ayurveda & Siddha and Unani systems of medicine. This herbal drug is referred to as...
A COMPREHENSIVE REVIEW ON COSTUS PICTUS D. DON
Costus pictus is rhizomatous medicinal herb, commonly known as “Insulin plant,” well known for its therapeutic properties. Many important and beneficial aspects of C. pictus are reported, which includes anti-diabetic, an...
SYNTHESIS, CHARACTERIZATION, CYTOTOXIC AND ANTIOXIDANT STUDIES OF MIXED LIGAND SCHIFF BASE COMPLEXES DERIVED FROM P- NITROANILINE AND 2, 4-DINITROANILINE
Four isatin mixed ligand complexes of the type ML1L2, where M =Co(II), Ni(II), Cu(II) and Zn(II) (LI = Schiff base formed by the condensation of isatin and p-nitroaniline and L2= Schiff base formed by the condensation o...
DEVELOPMENT AND VALIDATION OF A NEW HPLC-UV METHOD FOR SIMULTANEOUS ESTIMATION OF CETIRIZINE HYDROCHLORIDE AND PHENYLEPHRINE HYDROCHLORIDE IN TABLET FORMULATION
A simple, rapid, and sensitive high-performance liquid chromatographic method with UV detection has been developed and validated according to the ICH guidelines for the quantitation of Cetirizine Hydrochloride (CET) and...
PHARMACOKINETICS AND BIOEQUIVALENCE ASSESSMENT OF ORAL RIVAROXABAN TABLET IN IRANIAN HEALTHY VOLUNTEERS
Rivaroxaban is utilized as a direct factor Xa inhibitor for the prevention and remedy of thromboembolic disorders. This study aimed to evaluate a generic version of rivaroxaban 10 mg tablet. Considering previous reports...