Alteration of Testicular Macrophage Morphology and Associated Innate Immune Functions in Cadmium Intoxicated Swiss Albino Mice
Journal Title: Journal of Advances in Medicine and Medical Research - Year 2014, Vol 4, Issue 1
Abstract
Aims: The present study investigates in a mouse model the extent of immunomodulatory effects after exposure to cadmium chloride (in vivo) in the testes. Study Design: Experimental study. Place and Duration of Study: Department of Biotechnology, Assam University, Silchar, Assam, India; between may 2010 and march 2012. Methodology: LD50 was determined and the percent mortality of mice was plotted against their respective decreasing levels of cadmium chloride. To elucidate the immunomodulatory effects of cadmium chloride, Swiss albino mice were divided into two groups: the 1st group was intraperitonially injected with cadmium chloride (0.35 mg/kg b.w.) and the 2nd group with isotonic saline solution for 15 days. The isolated testicular macrophages were used to determine the morphological alteration as well as cell function studies such as phagocytosis, intracellular killing capacity, myeloperoxidase, nitric oxide release and TNF-α release assay from cadmium chloride -treated and control group of adult male Swiss albino mice. Results: The present work shows that cadmium chloride is responsible for a significant alteration in morphology from 22.2 ± 0.05% to 60.1 ± 1.19% (P**), degenerative changes in scanning electron microscopy and reduced cell function such as phagocytosis (from 21000 ± 577.35 to 7100 ± 115.47; P**), myeloperoxidase release (from 46.8 ± 0.872 µM to 30.23 ± 1.041 µM; P*), nitric oxide release (from 11 ± 1.53 to 5 ± 1.2; P*) and the intracellular killing capacity was also reduced significantly (P**) in testicular macrophages probably by increasing oxidative damage. It also shows that TNF-α increases with cadmium chloride treatment (from 164 ± 4.62 to 235 ± 5.2; P*). Conclusion: Thus it can be concluded that the toxic potential of cadmium chloride causes morphological changes as well as alterations in cell function in macrophages, rendering the animals more prone to infection, all of which may bear particular significance in heavy metal induced infertility.
Authors and Affiliations
Sumana Chakraborty, Mahuya Sengupta
Keywords
Related Articles
- EP ID EP348664
- DOI 10.9734/BJMMR/2014/5498
- Views 86
- Downloads 0
Sleep Duration the Night before an Exam and Its Relationship to Students’ Exam Scores
Background: Sleep is an important criterion for preserving physical and mental health. The amount and quality of our nightly sleep could affect cognitive functions during the following day’s activities. Recent reviews su...
Serum Levels of Interleukin-6 (IL-6) Cytokine in Patients with Classic Fever of Unknown Origin, in Maiduguri, Nigeria
Aim: To assess whether a significant correlation exists with serum Interleukin-6 cytokine levels in patients with Classic Fever of Unknown Origin (FUO) compared with healthy controls. The levels of IL-6 cytokine were tes...
Association between Anthropometric Indices, Plasma Insulin, Lipids and Lipoproteins in Overweight and Obese Nigerians
AIM: The aim of this study was to determine the relationship between excess body weight gain and plasma insulin, lipid profile and anthropometric indices in overweight/obese civil servants urban city dwellers in Nigeria...
Comparing Subcuticular Sutures versus Percutaneous Staples for Skin Closure after Caesarean Delivery: A Randomized Controlled Study
Background: Wound complications following caesarean delivery are significant causes of morbidity and financial strain especially in low resource countries. Use of appropriate technique in skin closure could reduce the em...
A Novel High-strength GIC System Composed of Poly (Acrylic Acid) With Different Molecular Architectures
Aims: The objective of this study was to synthesize and characterize the poly (acrylic acid) or PAA with different molecular architectures, use these polymers to formulate the cements with glass fillers, and evaluate the...