Alterations of NR1 expression and phosphorylation in the medial prefrontal cortex during neuropathic pain accompanied by negative emotions
Journal Title: Journal of Air Force Medical University - Year 2023, Vol 44, Issue 11
Abstract
Objective To study alterations of the NR1 subunit of N-methyl-D-aspartate receptors (NMDARs) in the expression and phosphorylation in medial prefrontal cortex (mPFC) brain region under the condition of neuropathic pain accompanied by negative emotions, and to provide a theoretical basis for exploring the involvement of NR1 in the occurrence and development of chronic pain accompanied by negative emotions. Methods Spared nerve injury modeling (SNI group) or Sham group were performed in C57BL / 6 male mice. Pain behaviors were detected at 7 and 28 d after modeling, and open field test, elevated plus maze test, and tail suspension test were detected at 28 d after modeling. Subsequently, proteins in mPFC brain region of mice were taken for the determination of NR1 expression and phosphorylation by Western blotting and immunofluorescence staining. Results Compared with the Sham group, SNI mice had decreased mechanical and thermal pain thresholds at 7 and 28 d and showed anxiety- and depression-like behaviors at 28 d, as well as significant upregulation of NR1 phosphorylation. Conclusion Under the condition of neuropathic pain accompanied by negative emotions, the phosphorylation of NR1 subunit of NMDARs in mPFC brain region was significantly upregulated, suggesting that NR1 in mPFC brain region may be involved in the occurrence and development of chronic pain accompanied by negative emotions.
Authors and Affiliations
TIAN Zhicheng, CAO Zixuan, LI Juan, WANG Fei, LIU Kexin, XU Lei, LI Haitao, LUO Peng, LUO Ceng,JIANG Xiaofan
Keywords
Related Articles
- EP ID EP724185
- DOI -
- Views 42
- Downloads 0
Analysis of 415 cases of endometrial cancer treated with Da Vinci robotic system
Objective To analyze the perioperative outcomes, clinicopathological characteristics and prognosis of the patients with endometrial cancer treated with Da Vinci robotic surgery system, and to investigate the safety, fea...
Study on the relationship between ribosome-binding protein 1 and prognosis of epithelial ovarian cancer
Objective To study the expression of ribosome-binding protein 1 (RRBP1) in epithelial ovarian cancer (EOC) and its relationship with clinical features and prognosis. Methods Tumor tissue specimens of 106 EOC patients w...
Protective effect of intranasal insulin on memory impairment in mice exposed to high altitude
Objective To explore the protective effect and mechanism of intranasal administration of insulin on memory impairment in mice exposed to high altitude. Methods Eighty female C57BL / 6 mice were randomly divided into no...
Risk factors of inadequate pain control after cesarean delivery under neuraxial anesthesia
Objective To analyze the risk factors of inadequate pain control in parturients who received neuraxial anesthesia and underwent patient-controlled intravenous analgesia ( PCIA) after cesarean delivery ( CD). Methods The...
Investigation of chronic prostatitis-like symptoms and related risk factors among soldiers at high altitude
Objective To investigate the incidence and related risk factors of chronic prostatitis-like symptoms (CPLS) in soldiers at high altitude. Methods A cluster sampling survey was conducted among soldiers at grass-roots lev...