Alternative Rapid Methods for Coverslip Removal: A Comparative Study
Journal Title: Journal of Clinical and Diagnostic Research - Year 2019, Vol 13, Issue 1
Abstract
ABSTRACT Introduction: In 21st century, the focus is to develop method which yields outcome in shorter tenure, and are rapid, safe and obtain quicker results. In this study, non-traditional methods were used for removal of coverslip and was compared with traditional methods. Aim: To compare the time taken for coverslip removal of old faded slides with five different methods. Materials and Methods: Faded slides of one year to more than 10-year-old were included in the study. Total of 90 slides were subjected to five different methods of coverslip removal like xylene at room temperature, xylene at 56° Celsius (C), Petrol, Diesel and Freezing method. Time taken for each method was noted. Results: The mean time taken for removal of coverslip was least with freezing method followed by xylene at 56°C, Petrol, xylene at room temperature and diesel. Conclusion: Alternative methods are safe, rapid and has less turn around time compared to routine use of xylene. Hence, can be successfully used for removal of coverslip.
Authors and Affiliations
Mahesh H Karigoudar, Sachin Shivaji Kapse, Disha B Shankar, Shikha Sharma, Divya Prafulla Yerraguntla
Keywords
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- EP ID EP557403
- DOI 10.7860/JCDR/2019/37367.12467
- Views 85
- Downloads 0
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