Alternative Splicing and Expression of Cattle Clusterin Gene in Healthy and Mastitis-Infected Mammary Tissues
Journal Title: Journal of Animal Science Advances - Year 2012, Vol 2, Issue 4
Abstract
The 1,369bp expressed clone sequence of clusterin (CLU) cDNA was used to evaluate transcriptional changes in the mammary tissue in Chinese Holstein Cattle. Two transcript variants (X-CLU and Y-CLU) were discovered in mastitis mammary tissue. X-CLU is the main transcript and Y-CLU is the splice transcript produced by the alternative 3' and 5' splice sites. Real-time PCR analysis confirmed the increased expression of X-CLU mRNA in mastitis mammary tissue (P<0.05). Western blot analysis showed that the clusterin gene in the Chinese Holstein cows had two distinct bands of about 60 kDa and 80 kDa in both normal and mastitis mammary tissues.
Authors and Affiliations
Huang J. ,Song L. , Ju Z. , Li Q. , Wang C. , Zhong J , Wang Z. , Xia P. , Wang G
Keywords
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