AMPHIBIAN SKIN SECRETIONS: A POTENTIAL SOURCE OF PROTEOLYTIC ENZYMES
Journal Title: Biotechnologia Acta - Year 2018, Vol 10, Issue 5
Abstract
The aim of the work was to study the protein content and proteolytic activity of the skin glands secretions of 10 the most common types of amphibians on the territory of Ukraine such as B. bombina, B. variegata, B. bufo, B. viridis, R. temporaria, P. ridibundus, P. esculentus, P. fuscus, S. salamandra, as well as the hybrid of B. bombina and B. variegata species. It was shown that the skin secretions of the studied amphibians contained a wide range of proteins with a molecular weight in the range from 8 to 150 kDa. By enzyme electrophoresis using gelatin, fibrinogen and collagen as substrates, it was found that they contained proteinases that differ in substrate specificity. It was revealed that the skin glands secretions of B. bombina, S. salamander species, as well as the hybrid of B. bombina and B. variegata species were characterized by he increased protein content with gelatinase and collagen activity.
Authors and Affiliations
I. Nikolaieva, Yu. Dudkina, D. Oliinyk, O. Oskyrko, T. Halenova
Keywords
Related Articles
- EP ID EP423780
- DOI 10.15407/biotech11.05.042
- Views 143
- Downloads 0
Russulaceae FAMILY MUSHROOMS LECTINS: FUNCTION, PURIFICATION, STRUCTURAL FEATURES AND POSSIBILITIES OF PRACTICAL APPLICATIONS
The purpose of this paper was to analise the results of own author’s research and literature date of other authors that concerned lectins of Russulaceae family mushrooms, which, despite their widespread, are still poorly...
ELECTRONIC INFORMATION SYSTEMS FOR MONITORING OF POPULATIONS AND MIGRATIONS OF INSECTS
The purpose of the work was to analyze existing information systems (IS) of biological objects and to propose the methods for development of such IS for insects on the example of Noctuidae (Lepidoptera). A detailed analy...
ISOLATION OF PURE CULTURES IRON- AND MANGANESE-OXIDIZING BACTERIA FROM RAPID FILTERS
The aim of the research was the isolation from drinking water the pure cultures of iron- and manganese-oxidizing microorganisms with further assessment of their efficacy to remove these contaminants on rapid filters. To...
DIAGNOSTIC CHARACTERISTICS OF THE ELISA TEST FOR THE HEPATITIS B VIRUS SURFACE ANTIGEN DETECTION
The aim of the work was to define the diagnostic ability of the enzyme immunoassay test system DIAHBsAg (PJSC “SPC “Diaprof-Med””), in which the principle of analysis is based on biotin-streptavidin amplification of a sp...
PRODUCTION AND APPLICATION OF ANGIOSTATINS FOR THE TREATMENT OF OCULAR NEOVASCULAR DISEASES
Angiostatins comprise a group of kringle-containing proteolytically-derived plasminogen/plasmin fragments, which act as potent inhibitory mediators of endothelial sells proliferation and migration. Angiostatins are invol...