Amplification efficiency and cost analysis of MP-NAT and ID-NAT in blood samples
Journal Title: Chinese Journal of Blood Transfusion - Year 2023, Vol 36, Issue 4
Abstract
Objective To compare the performance and cost-effectiveness between ID-NAT (individual detection of NAT) mode and MP-NAT (mini-pool nucleic acid test) mode in blood screening, and to provide reference for blood sample detection. Methods The results of 95 895 NAT-samples completed in 2021 were analyzed, and the Ct values of MP-NAT positive/ID-NAT negative group, MP-NAT positive/ID-NAT positive group, MP-NAT negative/ID-NAT positive group were compared. The amplification efficiency of MP-NAT and ID-NAT mode was estimated by PCR. Twelve samples from MP-NAT negative/ ID-NAT positive group were simulated by MP-NAT for 5 to 10 times. Results The Ct values of samples by MP-NAT were significantly higher than those by ID-NAT(P<0.05). The amplification efficiency of MP6-NAT was 0.16-0.71 times higher than that of ID-NAT. Among the 12 samples with MP-NAT-/ ID-NAT+, 11 samples were tested positive at least once after simulated 5 to 10 times by MP-NAT, and ELISA-HBsAg was 0.3<S/Co<Cutoff in 11 samples. Compared with MP-NAT, ID-NAT prolonged the run time of equipment by 3.5 times and increase the cost by 5.9 times. Conclusion The amplification efficiency of MP-NAT is lower than that of ID-NAT, and samples with lower viral load are prone to be missed in MP-NAT. However, the running-time and cost of ID-NAT are 3.5-5.9 times of those of MP-NAT.It is recommended to promote ID-NAT in areas with high HBV prevalence.
Authors and Affiliations
Ziyi HE, Guowei HAN, Qingkai CHEN, Qing WANG, Shaobin CHEN, Lin YU
Keywords
Related Articles
- EP ID EP738533
- DOI 10.13303/j.cjbt.issn.1004-549x.2023.04.013
- Views 73
- Downloads 0
Lymphocytopenia associated with leucocyte-reduced platelet apheresis: form concern, wane to concern again
In the early stage of the application of platelet apheresis technology, there was a concern that high-frequency platelet apheresis might lead to the risk of lymphocyte depletion in blood donors. With the advances in plat...
Quantitative detection of red blood cell antibody-mediated complement activation
Objective To construct an in-vitro model of erythrocyte antibody-mediated complement activation, and establish quantitative detection methods based on flow cytometry and spectrophotometry, so as to explore the correlatio...
Antigen distribution frequency of Han and Tujia polyhemia systems in Chongqing
[Objective] To analyse the distribution of antigen phenotypes in the Rh, MNS and Kidd blood group systems of Han and Tujia blood donors in Chongqing, and to provide data support for the establishment of an expanded blood...
A software for blood product information management: development and application
Objective To develop a mobile software application named " Component Assistant" and test for its performance in practical work, so as to address the difficulties and problems encountered during the management process of...
Bioinformatics analysis of HBV s gene mutation in blood donor
Objective To analyze the infectious indicators, HBV s gene mutations and changes in bioinformatics characteristics of HBV DNA+ /HBsAg- blood donor. Methods A sample of HBV DNA+ /HBsAg- was screened by PCR method, and HBs...