Amplification efficiency and cost analysis of MP-NAT and ID-NAT in blood samples
Journal Title: Chinese Journal of Blood Transfusion - Year 2023, Vol 36, Issue 4
Abstract
Objective To compare the performance and cost-effectiveness between ID-NAT (individual detection of NAT) mode and MP-NAT (mini-pool nucleic acid test) mode in blood screening, and to provide reference for blood sample detection. Methods The results of 95 895 NAT-samples completed in 2021 were analyzed, and the Ct values of MP-NAT positive/ID-NAT negative group, MP-NAT positive/ID-NAT positive group, MP-NAT negative/ID-NAT positive group were compared. The amplification efficiency of MP-NAT and ID-NAT mode was estimated by PCR. Twelve samples from MP-NAT negative/ ID-NAT positive group were simulated by MP-NAT for 5 to 10 times. Results The Ct values of samples by MP-NAT were significantly higher than those by ID-NAT(P<0.05). The amplification efficiency of MP6-NAT was 0.16-0.71 times higher than that of ID-NAT. Among the 12 samples with MP-NAT-/ ID-NAT+, 11 samples were tested positive at least once after simulated 5 to 10 times by MP-NAT, and ELISA-HBsAg was 0.3<S/Co<Cutoff in 11 samples. Compared with MP-NAT, ID-NAT prolonged the run time of equipment by 3.5 times and increase the cost by 5.9 times. Conclusion The amplification efficiency of MP-NAT is lower than that of ID-NAT, and samples with lower viral load are prone to be missed in MP-NAT. However, the running-time and cost of ID-NAT are 3.5-5.9 times of those of MP-NAT.It is recommended to promote ID-NAT in areas with high HBV prevalence.
Authors and Affiliations
Ziyi HE, Guowei HAN, Qingkai CHEN, Qing WANG, Shaobin CHEN, Lin YU
Keywords
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- EP ID EP738533
- DOI 10.13303/j.cjbt.issn.1004-549x.2023.04.013
- Views 34
- Downloads 0
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