An Aptamer-based Biosensor for Troponin I Detection in Diagnosis of Myocardial Infarction
Journal Title: Journal of Biomedical Physics and Engineering - Year 2018, Vol 8, Issue 2
Abstract
Background: Acute myocardial infarction (MI) accounts for one third of deaths. Cardiac troponin I (TnI) is a reliable biomarker of cardiac muscle tissue injury and is employed in the early diagnosis of MI. Objective: In this study, a molecular method is introduced to early diagnosis of MI by rapid detection of TnI. Materials and Methods: The detection method was based on electrochemical aptasensing, being developed using different methods and evaluation steps. A gold electrode was used as a transducer to successful immobilize 76base aptamer to fabricate a TnI biosensor. Results: The designed aptasensor could detect TnI in a range of 0.03 to 2.0 ng mL-1 without using any label, pre-concentration or amplification steps. The limit of detection was attained as 10 pg mL-1 without significant trouble of interfering species. The TnI biosensor demonestrated a stable, regenerative and reproducible function. 89 human samples were used to evaluate the performance of the TnI biosensor, and it represented 100% and 81%, diagnostic sensitivity and specificity, respectively. Conclusion: This aptasensor may be used as an applicable tool in the future of early medical diagnosis of MI.
Authors and Affiliations
M. Negahdary, N. Sattarahmady, H. Heli
Keywords
Related Articles
- EP ID EP453642
- DOI -
- Views 127
- Downloads 0
Comparative Analysis of Wavelet-based Feature Extraction for Intramuscular EMG Signal Decomposition
Background: Electromyographic (EMG) signal decomposition is the process by which an EMG signal is decomposed into its constituent motor unit potential trains (MUPTs). A major step in EMG decomposition is feature extracti...
Applications of Inertial Navigation Systems in Medical Engineering
Inertial navigation systems are of the most important and practical systems in determining the velocity, position and attitude of the vehicles and different equipment. In these systems, three accelerometers and three gyr...
Effects of Plantar Flexor Muscles Fatigue on Postural Control during Quiet Stance and External Perturbation in Healthy Subjects
Background: The maintenance of postural control is a key component in dynamic physical activity, especially during muscle fatigue and against external forces. Despite many studies in this field, there is no consensus reg...
Online Estimation of Elbow Joint Angle Using Upper Arm Acceleration: A Movement Partitioning Approach
Estimating the elbow angle using shoulder data is very important and valuable in Functional Electrical Stimulation (FES) systems which can be useful in assisting C5/C6 SCI patients. Much research has been conducted based...
Assessment of Adherence of Diagnostic Accuracy Studies Published in Radiology Journals to STARD Statement Indexed in Web of Science, PubMed & Scopus in 2015
Rationale and Objective: The objective of this study is to evaluate the methodological adherence of diagnostic accuracy studies published in radiology journals, which were indexed in different databases with the STARD st...