An Aptamer-based Biosensor for Troponin I Detection in Diagnosis of Myocardial Infarction
Journal Title: Journal of Biomedical Physics and Engineering - Year 2018, Vol 8, Issue 2
Abstract
Background: Acute myocardial infarction (MI) accounts for one third of deaths. Cardiac troponin I (TnI) is a reliable biomarker of cardiac muscle tissue injury and is employed in the early diagnosis of MI. Objective: In this study, a molecular method is introduced to early diagnosis of MI by rapid detection of TnI. Materials and Methods: The detection method was based on electrochemical aptasensing, being developed using different methods and evaluation steps. A gold electrode was used as a transducer to successful immobilize 76base aptamer to fabricate a TnI biosensor. Results: The designed aptasensor could detect TnI in a range of 0.03 to 2.0 ng mL-1 without using any label, pre-concentration or amplification steps. The limit of detection was attained as 10 pg mL-1 without significant trouble of interfering species. The TnI biosensor demonestrated a stable, regenerative and reproducible function. 89 human samples were used to evaluate the performance of the TnI biosensor, and it represented 100% and 81%, diagnostic sensitivity and specificity, respectively. Conclusion: This aptasensor may be used as an applicable tool in the future of early medical diagnosis of MI.
Authors and Affiliations
M. Negahdary, N. Sattarahmady, H. Heli
Keywords
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