AN ARRAY OF ENZYME BIOSENSORS FOR THE DETERMINATION OF CONCENTRATIONS OF NEUROTRANSMITTERS AND METABOLITES
Journal Title: Сенсорна електроніка і мікросистемні технології - Year 2018, Vol 15, Issue 2
Abstract
The purpose of this work was to test the possibility of creating an array of enzyme biosensors for the simultaneous determination of six substances in aqueous solutions. Enzymes, selective for glutamate, glucose, choline, acetylcholine, lactate and pyruvate, were immobilized on the surfaces of amperometric transducers. The influence of pH, ionic strength and buffer capacity of the solution on the response of biosensors was studied; the conditions of simultaneous operation of all bioselective elements were optimized. High selectivity of biosensors and absence of influence of interfering substances on their work was found. The created array of biosensors had good reproducibility of responses and storage stability. The use of identical transducers and similar conditions of immobilization of enzymes make the biosensor array promising for mass production. The biosensor array is suitable for simultaneous, rapid and simple determination of substances in aqueous samples.
Authors and Affiliations
D. Yu. Kucherenko, I. S. Kucherenko, O. O. Soldatkin, Ya. V. Topolnikova, D. V. Knyzhnykova, S. V. Dzyadevych, A. P. Soldatkin
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- EP ID EP399961
- DOI 10.18524/1815-7459.2018.2.136887
- Views 86
- Downloads 0
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