An easy and economical method of construction of tissue microarray
Journal Title: Scholars Journal of Applied Medical Sciences - Year 2016, Vol 4, Issue 11
Abstract
Abstract: Tissue microarray is very useful in research. However the automated equipment is very costly and cannot be afforded by many laboratories. We describe a very simple and cheap manual method of preparation of tissue microarray using readily available materials. Materials and methods: tissue cores were punched out from the donor blocks using a 16 g bone marrow trephine biopsy needle. The cores were aligned in a matrix of 6x5 on a transparent adhesive. L moulds were places around the matrix and molten paraffin wax was poured to form a paraffin block. Results: The block was then cut with a microtome and sections were used for immunohistochemistry and H & E. Excellent results were obtained from the method. Discussion: We have described a very simple and cheap method of construction of tissue microarray by using readily available materials, which does not require any special training or expertise. The method can be followed in any histopathology laboratory and its uses are many in the field of research, standardisation of antibodies and in situ hybridization. Keywords:tissue microarray, trephine biopsy needle, immunohistochemistry
Authors and Affiliations
DrAmit Kumar Adhya, DrNidhi Shukla
Keywords
Related Articles
- EP ID EP371329
- DOI -
- Views 73
- Downloads 0
Effective Treatment of a Dog with Insulinoma
Insulinoma is a rare neoplasm with a poor prognosis and few cases of healing in the world literature. A seven-years-old Dachshund presented with a history of spasms of the legs, the laboratory tests it was found hypoglyc...
The Association between Oral Health Habits and Quality of Life for Residents of a Psychiatric Nursing Home in Southern Taiwan
Abstract: The issue of daily care for patients with chronic mental disorders has recently been given more consideration. Many studies have revealed a strong association between oral diseases and chronic diseases. There i...
Comparative Study Betweensubcuticular and simple wound closure in incidence of wound infection
Abstract: Prevention of infection following surgery is important aim. Simple suture is most fundamental technique of wound closure used in. Another method ofwound closure is subcuticular stitches.subcuticular suture tech...
Assessment of Audiologic Evaluation in Patients with Acquired Hypothyroidism
Hypothyroidism is a common endocrine disorder resulting from deficiency of thyroid hormone or, more rarely, from their impaired activity at tissue level. In its clinically overt form, hypothyroidism is a relatively commo...
To Assess Skin Blood Flow Response to Breath Holding in Smokers
Abstract: Endothelial dysfunction causing pathologies associated with smoking alters blood flow to the skin. Skin blood flow is controlled through the release of compounds by vascular endothelium. The changes in peripher...