An Evaluation of the Sub Acute Toxicity and Haemostatic Effects of Leaves Extract of Achyranthes aspera in Mice and Albino Rats
Journal Title: European Journal of Medicinal Plants - Year 2015, Vol 7, Issue 1
Abstract
Background: Achyranthes aspera is used mostly in Nigerian and other folklore medicines to stop bleeding from wounds and in the treatment of other conditions such as dog bite, scorpion bite, gonorrhoea, obstetric disorders and diabetes mellitus. Objectives: The present study evaluated the toxicological and haemostatic effects of the leaves extract of Achyranthes aspera in adult Wistar albino rats and Swiss mice using experimental models. Methods: The acute toxicity profile of the methanol leaves extract, skin irritation test as well as the bleeding and clotting times in experimentally induced wounds were studied in Swiss mice and Wistar albino rats using standard methods. The phytochemical screening of the extract was undertaken using standard methods. Results: The intra-peritoneal LD50 of the extract was found to be 1224.7mg/kg and the topical skin irritation was negative. Phytochemical screening revealed the presence of alkaloids, saponins, tannins, flavonoids, anthraquinones, cardiac glycosides and terpenes. Administration of the extract for 21 days orally to the animals caused a significant (P=.05) decrease in clotting and bleeding times, with bleeding time in a dose-dependent manner compared to the control animals. Topical administration of the extract also caused a significant (P=.05) decrease in bleeding time compared to control animals with a less significant decrease when compared to orally administered extract. Conclusion: The results have shown that Achyranthes aspera, a traditional folklore medicinal plant has haemostatic effect which may provide therapeutic potential capable of arresting bleeding and contain some biologically active principle(s) which are responsible for the haemostasis.
Authors and Affiliations
Bassey Emmanuel Okon, Essien Ettienne Essien, Ching Fidelis Poh, Mbagwu Herbert Orji
Keywords
Related Articles
- EP ID EP350224
- DOI 10.9734/EJMP/2015/7043
- Views 145
- Downloads 0
Antibacterial Activity of Irradiated Powdered Tetrapleura tetraptera Fruit and the Moisture Sorption Isotherm of the Whole Fruit
Aim: To assess the microbial load and antibacterial activity of irradiated T. tetraptera fruit and the appropriate equilibrium relative humidity for the storage of irradiated fruit of T. tetraptera. Methodology: The whol...
Cytotoxicity of Various Fractions of Compounds Extracted from Yoyo Bitters on Human Cervical Cancer Cells
Aims: To investigate cytotoxicity of pure fractions of compounds extracted from yoyo bitters on human cervical cancer cell and to characterize the compounds present in various extracts. Study Design: Experimental Study...
Standardization of Amukkirai Chooranam, a Traditional Herbal Preparation in Siddha System of Medicine
Introduction: Amukkirai chooranam (A. chooranam) is an important traditional herbal preparation and its main component is the root powder of Withania somnifera Dunal (Solanaceae). It is the best remedy for rheumatism, we...
Evaluation of the Anticonvulsant Potential of Aqueous Fraction of Synsepalum dulcificum Seed Extract in Mice
Aim: This study evaluated the anticonvulsant potential of the aqueous fraction of Synsepalum ducificum seed extract in mice. This was with a view to providing information on the possible link between the antioxidant prin...
2, 2-Diphenyl-1-picrylhydrazyl Radical Effect and Phytochemical Constituents of Combretum platypetalum Welw. ex. M. A. Lawson subsp. oatesii (Rolfe) Exell Leaf
Aims: To investigate the phytochemical constituents and antioxidant properties of Combretum platypetalum leaf. Study Design: In vitro assessment of antioxidant properties and determination of the phytochemical constitue...