An experimental study on effect of antioxidant vitamin E in stress and alcohol induced changes in male fertility in albino rats
Journal Title: International Journal of Medical Research & Health Sciences (IJMRHS) - Year 2012, Vol 1, Issue 1
Abstract
Introduction: Physical and Psychological stresses are believed to reduce sexual functions, resulting from neurotransmission changes in various erectile response pathways and reduced blood flow in genital organs. Intake of alcohol depends on numerous genetic and environmental factors. Stress has long been thought to influence the alcohol drinking in humans. Forced swimming in laboratory animals has been widely used as a model of stress to study the physiological changes and the capacity of the organism in response to stress. Aim: 1. To assess the effect of forced swimming stress on seminal fluid profile, Serum testosterone level, Testicular lipid peroxidation levels. 2. To assess the effect of Vitamin E on stress induced changes. Method: Adult male albino rats weighing 200 – 220 g, aged 12-15 weeks were used in this study. The animals were randomly divided into four groups of 6 animals each. Group1 (control) received distilled water, Group 2 (Forced Swimming Stress) received distilled water Group 3: subjected to Forced Swimming stress with 20% alcohol intake p.o. Group 4: Swimming stress with 20% alcohol treated with Vitamin E (200mg/kg/day orally). The following parameters were studied in all groups 1. Body weight. 2. Sperm count Motility and Life /death Ratio (SLDR). 3. Serum Testosterone 4. Testicular Malondialdehyde level (MDA). Results: Forced swimming stress caused loss in body wt, reduction in sperm count, motility and SLDR in sperm analysis, reduction in serum testosterone and increase testicular MDA levels compared to control. All the changes were statistically significant. When alcohol was added along with forced swimming it caused further loss in body wt, sperm count, motility and SLDR, serum testosterone level and slightly further increase in MDA levels. These observations were also statistically significant. In case of group IV in which Vitamin E was administered long with stress with alcohol it showed a trend of reversal phenomenon of stress and alcohol induced change (Group III) exhibited by gain in body wt, increase sperm count, motility and SLDR, serum testosterone and decrease in MDA level. All the results were statically significant except serum testosterone. Conclusion: Force swimming indicates that swimming is an effective model for producing stress in albino rats. The stress induced changes were further accentuated by addition of ethyl alcohol. However Vit E treatment reverses the effect of stress and alcohol.
Authors and Affiliations
Sanghishetti Vijay Prasad| Department of Pharmacology, Padmashree Dr Vitthalrao Vikhe Pail Foundation’s Medical College, Ahmednagar, Maharashtra Corresponding author: Email:vijayfarmac@gmail.com, Ghongane BB| Department of Pharmacology, Govt. Medical College Miraj, Maharashtra, Nayak BB| Department of Pharmacology, Padmashree Dr Vitthalrao Vikhe Pail Foundation’s Medical College, Ahmednagar, Maharashtra, Raul AR| Department of Pharmacology, Padmashree Dr Vitthalrao Vikhe Pail Foundation’s Medical College, Ahmednagar, Maharashtra, Vijay Kumar AN| Department of Pharmacology, Padmashree Dr Vitthalrao Vikhe Pail Foundation’s Medical College, Ahmednagar, Maharashtra, Mutalik MM| Department of Pharmacology, Padmashree Dr Vitthalrao Vikhe Pail Foundation’s Medical College, Ahmednagar, Maharashtra, Kapure NL| Department of Pharmacology, Padmashree Dr Vitthalrao Vikhe Pail Foundation’s Medical College, Ahmednagar, Maharashtra
Keywords
Related Articles
- EP ID EP10938
- DOI -
- Views 532
- Downloads 27
A study to find out the immediate effect of macquarie injury management group (mimg) protocol on pain and range of motion in primary osteoarthritis knee patients- an interventional study
Osteoarthritis is a disorder of the diarthrodial joint, a slow degenerative disease clinically characterized by pain, loss of range of motion. On a radiograph seen as reduced joint space, formation of osteophytes, scler...
Iranian key informants' perspectives towards gender sensitive STIs/HIV/AIDS prevention service delivery
More than 340 million new cases of sexually transmitted infections occur throughout the world every year. The average annual STIs growth rate was 17%from 1998 to 2006 in Iran. As this epidemic has progressed, relevant...
Integrated intensive proprioceptive and visuomotor rehabilitation program for treatment of spastic diplegic Children
Although so many rehabilitation programs have been addressed for rehabilitation of diplegic children, it still a challenging task to attain a satisfactory functional recovery. The purpose of the study was to investigate...
Evaluation of emergency department performance improvement-A systematic review on influence factors
The emergency department as entrance of hospital and in close relationship with other sections of the emergency service providers provides the wide range of services from the stage of acceptance and initial assessment t...
Comparative diagnosis of urinary tract infection (UTI) using urinary nitrite and significant bacteriuria (SBU)
The clinical laboratory diagnosis of urinary tract infection was compared in two hundred (200) midstream urine samples using bacteria culture and urinary nitrite detection technique. The comparative susceptibility of th...