An HPLC Method for the Determination of Saxagliptin in Human Plasma with Fluorescence Detection
Journal Title: JOURNAL OF ADVANCES IN CHEMISTRY - Year 2013, Vol 5, Issue 3
Abstract
A sensitive and selective HPLC method with fluorometric detection was developed for the determination of saxagliptin (SGX) in human plasma and applied to a pharmacokinetic study. SGX was precolumn derivatized with fluorescamine, and the fluorescent derivative was separated on an RP C18 column using a mobile phase composed of acetonitrile-10 mM orthophosphoric acid by isocratic elution with flow rate of 1.3 ml/min. The method was based on the measurement of the derivative using fluorescence detection at 378 nm, with excitation at 463 nm. The calibration curve was linear over the range of 3.0“100.0 ng/ml. LOD and LOQ were found to be 0.15 and 0.5 ng/ml, respectively. Intraday and interday RSD values were less than 2.84%. The plasma concentration“time profile and pharmacokinetic parameters such as AUC0–t, AUC0–∞, Cmax, tmax, t1/2, were calculated according to the assays.
Authors and Affiliations
Serife Evrim Kepekci Tekkeli, Mustafa Volkan K?z?lta?, Demet Dincel
Keywords
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- EP ID EP652593
- DOI 10.24297/jac.v5i3.2678
- Views 179
- Downloads 0
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